Rabbit Anti-SLC5A1 antibody

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D22S675; High affinity sodium glucose cotransporter 1; GLT1; GLT-1; High affinity sodium glucose cotransporter; Human Na+/glucose cotransporter 1; Na(+)/glucose cotransporter 1; NAGT; SGLT 1; SGLT1; Sodium glucose cotransporter 1; Sodium/glucose cotranspo

Cat:

SL1128R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human SGLT1:401-500/665

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Mouse Kidney Lysates Lane 2: Human 293T cell Lysates Lane 3: Human HepG2 cell Lysates Primary: Anti-SLC5A1 (SL1128R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 73kDaObserved band size: 70kDaParaformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SGLT1) Polyclonal Antibody, Unconjugated (SL1128R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embeddedAntigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15minBlock endogenous peroxidase by 3% Hydrogen peroxide for 30minBlocking buffer (normal goat serum) at 37℃ for 20 minIncubation: Anti-SGLT1 Polyclonal Antibody, Unconjugated(SL1128R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Function:
Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed mainly in intestine and kidney.

Post-translational modifications:
N-glycosylation is not necessary for the cotransporter function.

DISEASE:
Congenital glucose/galactose malabsorption (GGM) [MIM:606824]: Intestinal monosaccharide transporter deficiency. It is an autosomal recessive disorder manifesting itself within the first weeks of life. It is characterized by severe diarrhea and dehydration which are usually fatal unless glucose and galactose are eliminated from the diet. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

SWISS:
Q8C3K6

Gene ID:
6523

Database links:

Entrez Gene: 6523 Human

Entrez Gene: 20537 Mouse

Entrez Gene: 397113 Pig

Entrez Gene: 25552 Rat

Omim: 18476 Human

SwissProt: P13866 Human

SwissProt: Q8C3K6 Mouse

SwissProt: P53790 Rat

Unigene: 1964 Human

Unigene: 25237 Mouse

Unigene: 10224 Rat

葡萄糖的跨膜转运主要是通过SGLT1结合1 mol葡萄糖,2 mol的Na+,形成Na+-载体-葡萄糖复合物,顺Na+的浓度梯度进入细胞.不同物种的SGLT1具有较高的同源性. Picture

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SGLT1) Polyclonal Antibody, Unconjugated (SL1128R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min Block endogenous peroxidase by 3% Hydrogen peroxide for 30min Blocking buffer (normal goat serum) at 37℃ for 20 min Incubation: Anti-SGLT1 Polyclonal Antibody, Unconjugated(SL1128R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

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