Rabbit Anti-Cyclin G antibody

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CCNG 1; CCNG; CCNG1; CYCG1; Cyclin G; CCNG1_HUMAN; Cyclin-G; Cyclin G1; CyclinG1; Cyclin-G1.

Cat:

SL1389R

Species Reactivity：

Human,Mouse,(predicted: Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human Cyclin G:181-295/295

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Liver (Mouse) Lysate at 40 ugPrimary: Anti-Cyclin G (SL1389R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 34 kDObserved band size: 34/30 kDSample: Kidney (Mouse) Lysate at 40 ugPrimary: Anti-Cyclin G (SL1389R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 34 kDObserved band size: 34/30 kDSample: Spleen (Mouse) Lysate at 40 ugPrimary: Anti-Cyclin G (SL1389R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 34 kDObserved band size: 34/30 kDTissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Cyclin G Polyclonal Antibody, Unconjugated(SL1389R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingHepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (Cyclin G) polyclonal Antibody, Unconjugated (SL1389R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Cyclin G contains a typical N terminal cyclin box and a carboxy terminal domain sequence homologous to the tyrosine phosphorylation site of the epidermal growth factor receptor. Cyclin G2 shares 53% amino acid sequence identity with cyclin G1. Peak expression of cyclin G2 is seen in late S phase, as opposed to cyclin G1 expression, which is constitutive.

Function:
May play a role in growth regulation. Is associated with G2/M phase arrest in response to DNA damage. May be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation (By similarity).

Subcellular Location:
Nucleus. DNA replication foci after DNA damage.

Tissue Specificity:
High levels in skeletal muscle, ovary, kidney and colon.

Similarity:
Belongs to the cyclin family. Cyclin G subfamily.

SWISS:
P51959

Gene ID:
900

Database links:

Entrez Gene: 900 Human

Omim: 601578 Human

SwissProt: P51959 Human

Unigene: 79101 Human

Cyclin G的生物功能还没有完全清楚，他是细胞周期蛋白家族中近来发现的新成员，也是已知的唯一受到肿瘤抑制基因p53调控的细胞周期蛋白。
近年来经研究发现，Cyclin G可能有促进细胞增长的作用，在细胞增殖和神经再生中的可能起到一定的作用。 Picture

Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-Cyclin G (SL1389R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34/30 kD

Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Cyclin G (SL1389R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34/30 kD

Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Cyclin G Polyclonal Antibody, Unconjugated(SL1389R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (Cyclin G) polyclonal Antibody, Unconjugated (SL1389R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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