This protein was identified in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this protein has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70 kDa component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this protein suggests that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling.
Function:
May be involved in the regulation of gene expression by covalent modification of histone proteins. Isoform Long is a corepressor of estrogen receptor (ER). Isoform Short binds to ER and sequesters it in the cytoplasm and enhances non-genomic responses of ER.
Subunit:
Component of the nucleosome-remodeling and histone-deacetylase multiprotein complex (NuRD). Interacts with HDAC1 and ITGB3BP/CENPR. Binds to CSNK1G2 in the cytoplasm.
Subcellular Location:
Cytoplasm and Nucleus.
Tissue Specificity:
Widely expressed. High expression in brain, ovaries, adrenal glands and virgin mammary glands. Higher in tumors than in adjacent normal tissue from the same individual.
Similarity:
Contains 1 BAH domain.
Contains 1 ELM2 domain.
Contains 1 GATA-type zinc finger.
Contains 1 SANT domain.
SWISS:
Q13330
Gene ID:
9112
Database links:
Entrez Gene: 9112 Human
Entrez Gene: 116870 Mouse
Entrez Gene: 64520 Rat
Omim: 603526 Human
SwissProt: Q13330 Human
SwissProt: Q2KHS8 Mouse
SwissProt: Q8K4B0 Mouse
SwissProt: Q62599 Rat
Unigene: 101448 Human
Unigene: 525629 Human
Unigene: 212577 Mouse
Unigene: 5168 Rat
转录调节因子（Transcriptin Regulators）
MTA1(Metastasis-associated 1)蛋白是一种蛋白复合物,它具有组蛋白脱乙酰基酶的活性,在组蛋白的脱乙酰基,染色质重塑,转录控制的过程中具有重要作用。
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Sample: Lung(Mouse) Lysate at 30 ug
Primary: Anti- MTA1 (SL1412R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 81 kD
Observed band size: 85 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTA1) Polyclonal Antibody, Unconjugated (SL1412R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTA1) Polyclonal Antibody, Unconjugated (SL1412R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-MTA1 Polyclonal Antibody, Unconjugated(SL1412R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: transplanted tumor of cervical carcinoma in nude mice; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-MTA1 Polyclonal Antibody, Unconjugated(SL1412R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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