Rabbit Anti-MTNR1B antibody

Home > Product > Antibody > Rabbit Anti-MTNR1B antibody

MT2; melatonin receptor type 1B; melatonin receptor; MEL1B; MEL-1B-R; Mtnr1b; MGC129286;MGC129287; MTNR-1B; Melatonin receptor 1B; MTR1B_HUMAN.

Cat:

SL0963R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,)

Immunogen：

KLH conjugated synthetic peptide derived from human MTNR1B:201-300/362<Cytoplasmic>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ugLane 2: Cerebellum (Mouse) Lysate at 40 ugLane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ugLane 4: Cerebrum (Rat) Lysate at 40 ugLane 5: Cerebellum (Rat) Lysate at 40 ugLane 6: Jurkat (Human) Cell Lysate at 30 ugLane 7: Hela (Human) Cell Lysate at 30 ugLane 8: U87MG (Human) Cell Lysate at 30 ugPrimary: Anti-MTNR1B (SL0963R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 40/50-55 kDObserved band size: 60/50 kDSample: HepG2(Human) Cell Lysate at 30 ugPrimary: Anti- MTNR1B (SL0963R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 40 kDObserved band size: 40 kDParaformaldehyde-fixed, paraffin embedded (Mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTNR1B) Polyclonal Antibody, Unconjugated (SL0963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTNR1B) Polyclonal Antibody, Unconjugated (SL0963R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat retina tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-MTNR1B Polyclonal Antibody, Unconjugated(SL0963R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-MTNR1B Polyclonal Antibody, Unconjugated(SL0963R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingHela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (MTNR1B) polyclonal Antibody, Unconjugated (SL0963R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

prev next

Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin.

Function:
High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed in retina and less in brain and hippocampus.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P49286

Gene ID:
4544

Database links:

Entrez Gene: 4544 Human

Omim: 600804 Human

SwissProt: P49286 Human

Unigene: 569039 Human

有学者认为：MTNR1B是组成控制褪黑激素信号通路的一部分。
褪黑激素调解人体的昼夜节律，是通过白天和黑夜来控制睡眠和饮食的内部时钟。MTNR1B的进一步研究为高血糖和糖尿病与昼夜节律遭到破坏有直接关系这一观点提供了证据。 Picture

Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti- MTNR1B (SL0963R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 40 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTNR1B) Polyclonal Antibody, Unconjugated (SL0963R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTNR1B) Polyclonal Antibody, Unconjugated (SL0963R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: rat retina tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-MTNR1B Polyclonal Antibody, Unconjugated(SL0963R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-MTNR1B Polyclonal Antibody, Unconjugated(SL0963R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (MTNR1B) polyclonal Antibody, Unconjugated (SL0963R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Product Feedback Wall

Specific References (8) | SL0963R has been referenced in 8 publications.

[IF=5.546] Donghui Yang. et al. Melatonin alleviates LPS‐induced endoplasmic reticulum stress and inflammation in spermatogonial stem cells. J Cell Physiol. 2021 May;236(5):3536-3551 WB,IF,IHC ; Mouse.

PubMed:32996162

[IF=1.778] Zhao Shu-Qin. et al. The in vitro effects of melatonin and Cry gene on the secretion of estradiol from camel ovarian granulosa cells. Domest Anim Endocrin. 2021 Jan;74:106497 WB,IF ; Camel.

PubMed:32799039

[IF=1.778] S.-Q. Zhao. et al. cAMP/PKA/CREB signaling pathway-mediated effects of melatonin receptor genes on clock gene expression in Bactrian camel ovarian granulosa cells. Domest Anim Endocrin. 2021 Jul;76:106609 IHC ; Camel.

PubMed:33636446

[IF=10.39] Jang, Hoon, et al. "Synergistic effect of melatonin and ghrelin in preventing cisplatin‐induced ovarian damage via regulation of FOXO3a phosphorylation and binding to the p27Kip1 promoter in primordial follicles." Journal of Pineal Research (2017). IHSLCP ; Mouse.

PubMed:28658519

[IF=2.17] Brockus, K. E., et al. "Dietary melatonin alters uterine artery hemodynamics in pregnant Holstein heifers." Domestic Animal Endocrinology (2015). Bovine.

PubMed:26641925

[IF=1.291] Gao Y et al. Melatonin receptor depletion suppressed hCG-induced testosterone expression in mouse Leydig cells.Cell Mol Biol Lett. 2019 Mar 12;24:21. IHSLCP,IF&WB ; Mouse.

PubMed:30915128

[IF=2.478] Tabecka-Lonczynska A et al. Melatonin receptors subtypes (MT1 and MT2) in the uterus masculinus of mature male european bison. Biological and seasonal reproductive role.J Physiol Pharmacol. 2018 Feb;69(1):67-73. WB ; European bisons.

PubMed:29769422

[IF=2.08] Wang, S-J., et al. "Melatonin suppresses apoptosis and stimulates progesterone production by bovine granulosa cells via its receptors (MT1 and MT2)." Theriogenology 78.7 (2012): 1517-1526. WB ; Bovine.

PubMed:2596085