Rabbit Anti-LPLUNC1 antibody

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BPI fold containing family B, member 1; long palate, lung and nasal epithelium carcinoma associated 1 isoform 1; chromosome 20 open reading frame 114; C20orf114; Long palate; BPIB1_HUMAN; Lplunc1; lung and nasal epithelium carcinoma-associated protein 1;

Cat:

SL0976R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from human LPLUNC1:21-120/484

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Stomach (Mouse) Lysate at 40 ugPrimary: Anti- LPLUNC1 (SL0976R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50 kDObserved band size: 50 kDSample: Jurkat(Human) Cell Lysate at 30 ugPrimary: Anti- LPLUNC1 (SL0976R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 50 kDObserved band size: 50 kDSample: Lane1: Lung(Rat) Lysate at 30 ugLane2:Colon carcinoma(Human) Lysate at 30 ugPrimary: Anti-LPLUNC1 (SL0976R) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution; Predicted band size: 50kDObserved band size: 53kDTissue/cell: human Nasal polyps tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-LPLUNC1 Polyclonal Antibody, Unconjugated(SL0976R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene may be involved in the innate immune response to bacterial exposure in the mouth, nasal cavities, and lungs. The encoded protein is secreted and is a member of the BPI/LBP/PLUNC protein superfamily. This gene is found with other members of the superfamily in a cluster on chromosome 20. [provided by RefSeq]

Function:
May play a role in innate immunity in mouth, nose and lungs.

Subcellular Location:
Secreted (By similarity).

Tissue Specificity:
Detected in trachea, nasal septal epithelium and lung.

Similarity:
Belongs to the BPI/LBP/Plunc superfamily. Plunc family.

SWISS:
Q8TDL5

Gene ID:
92747

Database links:

Entrez Gene: 92747 Human

SwissProt: Q8TDL5 Human

Unigene: 65551 Human

Picture

Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti- LPLUNC1 (SL0976R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD

Sample:
Lane1: Lung(Rat) Lysate at 30 ug
Lane2:Colon carcinoma(Human) Lysate at 30 ug
Primary: Anti-LPLUNC1 (SL0976R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution;
Predicted band size: 50kD
Observed band size: 53kD

Tissue/cell: human Nasal polyps tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-LPLUNC1 Polyclonal Antibody, Unconjugated(SL0976R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

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