Rabbit Anti-NQO1 antibody | Sunlong Medical

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Azoreductase; Cytochrome b 5 reductase; DHQU; DIA 4; DIA4; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase); Diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase (NADH/NADPH); Diaphorase 4; D

Cat:

SL2184R

Species Reactivity：

Human,Mouse,Rat,(predicted: Pig,Horse,Rabbit,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human NQO1:201-274/274

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ugLane 2: HepG2 (Human) Cell Lysate at 30 ugLane 3: MCF-7 (Human) Cell Lysate at 30 ugLane 4: Du145 (Human) Cell Lysate at 30 ugPrimary: Anti-NQO1 (SL2184R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 31 kDObserved band size: 31 kDSample: A549(Human) Cell Lysate at 30 ugPrimary: Anti-NQO1 (SL2184R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 31 kDObserved band size: 31 kDSample: Lane1: Liver(Rat) Lysate at 30 ugLane2: Brain(Rat) Lysate at 30 ugPrimary: Anti-NQO1 (SL2184R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution; Predicted band size : 31kDObserved band size : 30kDParaformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NQO1) Polyclonal Antibody, Unconjugated (SL2184R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: mouse colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-NQO1 Polyclonal Antibody, Unconjugated(SL2184R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].

Function:
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.

Subunit:
Homodimer.

Subcellular Location:
Cytoplasm.

Similarity:
Belongs to the NAD(P)H dehydrogenase (quinone) family.

SWISS:
P15559

Gene ID:
1728

Database links:

Entrez Gene: 610935 Dog

Entrez Gene: 1728 Human

Entrez Gene: 18104 Mouse

Entrez Gene: 100286873 Pig

Entrez Gene: 24314 Rat

Omim: 125860 Human

SwissProt: P15559 Human

SwissProt: Q64669 Mouse

SwissProt: P05982 Rat

Unigene: 406515 Human

Unigene: 252 Mouse

Unigene: 11234 Rat

合成与降解（Synthesis and Degradation）
醌氧化还原酶（NQO1）是一种黄素酶，它催化醌双电子还原反应，以减少氧自由基的产生。经研究发现NQO1可能有抗氧化效应，目前多用于帕金森病（PD）和部分肿瘤方面的研究。
醌是一种有毒的化合物,能诱发哺乳动物细胞癌变、突变和坏死。 Picture

Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-NQO1 (SL2184R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD

Sample:
Lane1: Liver(Rat) Lysate at 30 ug
Lane2: Brain(Rat) Lysate at 30 ug
Primary: Anti-NQO1 (SL2184R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution;
Predicted band size : 31kD
Observed band size : 30kD

Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NQO1) Polyclonal Antibody, Unconjugated (SL2184R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: mouse colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-NQO1 Polyclonal Antibody, Unconjugated(SL2184R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Specific References (16) | SL2184R has been referenced in 16 publications.

[IF=6.953] Jinshan Wu. et al. Protection by Hosta ventricosa polysaccharides against oxidative damage induced by t-SHP in HepG2 cells via the JNK/Nrf2 pathway. Int J Biol Macromol. 2022 May;208:453 WB ; Human.

PubMed:35339497

[IF=1.07] Liu, Cui-Zhen, et al. "HBSP attenuates lipopolysaccharide-induced inflammatory response in human renal proximal tubular epithelial cells by induction of Nrf2 via PI3K/Akt pathway." Int J Clin Exp Med 10.1 (2017): 587-597. WB ; Human.

PubMed:ISSN:1940-5901/IJCEM0037069

[IF=2.571] Tang et al. Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways. (2016) Cell.Stress.Chaperones. 21:239-49 WB ; Rat.

PubMed:26577463

[IF=3.909] Wang X et al. Sarcodon imbricatus polysaccharides protect against cyclophosphamide-induced immunosuppression via regulating Nrf2-mediated oxidative stress.(2018) Int. J. Biol. Macromol.Dec;120(Pt A):736-744. WB ; Mouse.

PubMed:30171947

[IF=6.291] Xiao Wang. et al. Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis. Ecotox Environ Safe. 2022 Mar;233:113339 WB ; Chicken.

PubMed:35219255

[IF=6.081] Naoki Yoshida. et al. Relationship between Cognitive Dysfunction and Age-Related Variability in Oxidative Markers in Isolated Mitochondria of Alzheimer’s Disease Transgenic Mouse Brains. Biomedicines. 2022 Feb;10(2):281 WB ; Mouse.

PubMed:10.3390/biomedicines10020281

[IF=3.06] Wang, Huihui. et al. Anti-inflammatory and Antioxidant Effects of Pyrroloquinoline Quinone in L-NAME-Induced Preeclampsia-Like Rat Model. 2021 Sep 20 WB ; Rat.

PubMed:10.1007/s43032-021-00743-8

[IF=1.641] Feng Gao. et al. Aucubin ameliorates the LPS‐induced inflammatory response in bovine endometrial epithelial cells by inhibiting NF‐κB and activating the Keap1/Nrf2 signalling pathway. 2021 Apr 27 WB ; Bovine.

PubMed:33866621

[IF=2.55] Shen, Haitao, et al. "Chloroquine attenuates paraquat-induced lung injury in mice by altering inflammation, oxidative stress and fibrosis." International Immunopharmacology 46 (2017): 16-22. WB ; Mouse.

PubMed:28249220

[IF=3.83] Wang, Peng, et al. "Geraniin exerts cytoprotective effect against cellular oxidative stress by upregulation of Nrf2-mediated antioxidant enzymes expression via PI3K/AKT and ERK1/2 pathway." Biochimica et Biophysica Acta (BBA)-General Subjects (2015). WB ; Human.

PubMed:25917210

[IF=6.992] Qingyue Yang. et al. The link between deacetylation and hepatotoxicity induced by exposure to hexavalent chromium. J Adv Res. 2021 Apr;: WB ; Rat.

PubMed:10.1016/j.jare.2021.04.002

[IF=3.056] Xiao Wang. et al. Florfenicol induces renal toxicity in chicks by promoting oxidative stress and apoptosis. Environ Sci Pollut R. 2021 Jan;28(1):936-946 WB ; Chicken.

PubMed:32827115

[IF=5.076] Zhang Yifanet al. Ceftriaxone Calcium Crystals Induce Acute Kidney Injury by NLRP3-Mediated Inflammation and Oxidative Stress Injury. Oxid Med Cell Longev . 2020 Jul 9;2020:6428498. WB ; Human.

PubMed:32695257

[IF=4.527] Han C et al. Florfenicol induces oxidative stress and hepatocyte apoptosis in broilers via Nrf2 pathway. Ecotoxicol Environ Saf. 2020 Mar 15;191:110239. WB ; Chicken.

PubMed:31991393

[IF=5.97] Zhang et al. Nrf2 antioxidant pathway suppresses Numb-mediated epithelial-mesenchymal transition during pulmonary fibrosis. (2018) Cell.Death.Dis. 9:83 WB ; Mouse, Rat, Human.

PubMed:29362432

[IF=2.4] Zhao, Feng, et al. "Silymarin attenuates paraquat‐induced lung injury via Nrf2‐mediated pathway in vivo and in vitro." Clinical and Experimental Pharmacology and Physiology (2015). WB ; Human.

PubMed:26173462