Rabbit Anti-SHBG antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-SHBG antibody

Androgen binding protein; ABP; MGC126834; MGC138391; SBP; Sex steroid binding protein; SHBG; TEBG; Testis specific androgen binding protein; Testosterone binding beta globulin; Testosterone Estradiol Binding Globulin; Testosterone estrogen binding globuli

Cat:

SL2410R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Cow,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human SHBG:51-150/402

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Human HepG2 cell lysates Lane 2: Human U87MG cell lysates Primary: Anti-SHBG (SL2410R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 40 kDaObserved band size: 50 kDaSample: Lane1: Testis (Mouse) Lysate at 30 ugLane2: Liver (Mouse) Lysate at 30 ugPrimary: Anti-ABP/SHBG(SL2410R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution; Predicted band size : 40kDObserved band size : 44kDParaformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (SL2410R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (SL2410R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (SL2410R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

prev next

Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Sex hormone binding globulin (SHBG) is a glycoprotein synthesized by the liver. Circulating androgen and estrogen concentrations influence SHBG synthesis. Elevated testosterone, for example, causes SHBG synthesis to decrease, whereas high estrogen stimulates SHBG production. The regulation of SHBG synthesis, combined with SHBG's higher affinity for testosterone, impacts bioavailable testosterone levels. SHBG binds up to 98 percent of the steroid hormones in the blood including 5a-dihydrotestosterone (DHT), testosterone and androstenediol with particularly high affinity, and estradiol and estrone with slightly lower affinity.

Function:
Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.

Subunit:
Homodimer.

Subcellular Location:
Secreted (By similarity). Note=In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis (By similarity).

Tissue Specificity:
Isoform 1 and isoform 2 are present in liver and testis.

Similarity:
Contains 2 laminin G-like domains.

SWISS:
P04278

Gene ID:
6462

Database links:

Entrez Gene: 404182 Cow

Entrez Gene: 6462 Human

Omim: 182205 Human

SwissProt: P04278 Human

Unigene: 632235 Human

雄激素结合蛋白ABP是一种由睾丸支持细胞合成和分泌的与雄激素有高度亲和力的蛋白,雄激素是依赖于雄激素结合蛋白起作用的,ABP与睾酮或双氢睾酮结合后，转运至曲细精管，提高雄激素在曲细精管的局部浓度，有利于生精过程。 Picture

Sample:
Lane1: Testis (Mouse) Lysate at 30 ug
Lane2: Liver (Mouse) Lysate at 30 ug
Primary: Anti-ABP/SHBG(SL2410R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution;
Predicted band size : 40kD
Observed band size : 44kD

Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (SL2410R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABP) Polyclonal Antibody, Unconjugated (SL2410R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Product Feedback Wall