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Home > Product > Antibody > Rabbit Anti-CD63 antibody
Lysosomal associated membrane protein 3; CD63 antigen; CD63 antigen melanoma 1 antigen; CD63 molecule; granulophysin; LAMP 3; LAMP3; lysosome associated membrane glycoprotein 3; CD 63; CD63 antigen (melanoma 1 antigen); CD63_HUMAN; gp55; granulophysin; LA
Cat:
SL1523R
Species Reactivity：
Human,
Immunogen：
KLH conjugated synthetic peptide derived from human CD63:101-200/238<Extracellular>
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample:A431 cell(human) Lysate at 40 ugHL-60 cell(human) Lysate at 40 ugMCF-7 cell(human) Lysate at 40 ugU87mg cell(human)Lysate at 40 ugPrimary: Anti- CD63 (SL1523R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 26kDObserved band size: 53 kD Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-CD63 Polyclonal Antibody, Unconjugated(SL1523R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingcell: A549 cellsIncubation: Avoid light, at 4°C for 60 minutes. Blank control A549 cells(red line),5X10^5/ml, at 4°C for 60 minutes. primary antibody: abbit Anti-CD63 antibody (SL1523R-PE,Blue line) ,1:50, at 4°C for 60 minutes.Blank control: Hep G2 cells(blue). Primary Antibody:Rabbit Anti- CD23 antibody(SL1523R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.ProtocolThe cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (SL1523R,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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Product PDFs
Datasheet:


The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

Function:
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.

Subcellular Location:
Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Note=Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.

Tissue Specificity:
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.

Similarity:
Belongs to the tetraspanin (TM4SF) family.

SWISS:
P08962

Gene ID:
967

Database links:

Entrez Gene: 967 Human

Omim: 155740 Human

SwissProt: P08962 Human

Unigene: 445570 Human



CD63为溶酶体膜糖蛋白也是一种细胞表面糖蛋白，可表达在多种细胞的表面和胞浆内，血小板活化后即转移至膜表面。
CD63—又称黑色素瘤相关抗原MLA1和整合素形成复合物。是血液血小板活化的标志。与一些肿瘤的进展也有关联。 Picture

Sample:
A431 cell(human) Lysate at 40 ug
HL-60 cell(human) Lysate at 40 ug
MCF-7 cell(human) Lysate at 40 ug
U87mg cell(human)Lysate at 40 ug
Primary: Anti- CD63 (SL1523R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26kD
Observed band size: 53 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CD63 Polyclonal Antibody, Unconjugated(SL1523R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
cell: A549 cells
Incubation: Avoid light, at 4°C for 60 minutes.
Blank control A549 cells(red line),5X10^5/ml, at 4°C for 60 minutes.
primary antibody: abbit Anti-CD63 antibody (SL1523R-PE,Blue line) ,1:50, at 4°C for 60 minutes.
Blank control: Hep G2 cells(blue).
Primary Antibody:Rabbit Anti- CD23 antibody(SL1523R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (SL1523R,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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