Rabbit Anti-COX4I1 antibody

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COX41_HUMAN; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX4; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1 (COX IV-1); COX-IV; COX IV; COX 4; COX 4I1;

Cat:

SL1533R

Species Reactivity：

Human,Mouse,(predicted: Rat,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human COX4I1:101-169/169

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: kidney (Mouse) Lysate at 40 ug Primary: Anti-COX4 (Bs-1533R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 17 kDSample: heart (Mouse) Lysate at 40 ugPrimary: Anti-COX4 (Bs-1533R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 17 kDObserved band size: 17 kDTissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-COX4/COX ISLV1 Polyclonal Antibody, Unconjugated(SL1533R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingPositive control: Hela cellsConcebtration: 5μg/10^6 cellsIncubation conditions: Avoid light , 30 minutes on the ice.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq, Jul 2008]

Function:
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

Subcellular Location:
Mitochondrion inner membrane.

Tissue Specificity:
Ubiquitous.

Similarity:
Belongs to the cytochrome c oxidase IV family.

SWISS:
P13073

Gene ID:
1327

Database links:

Entrez Gene: 1327 Human

Entrez Gene: 12857 Mouse

Entrez Gene: 29445 Rat

SwissProt: P13073 Human

SwissProt: P19783 Mouse

SwissProt: P10888 Rat

细胞色素c氧化酶4亚型1抗体；（细胞色素c氧化酶IV亚型1）是一种存在线粒体上的大型跨膜蛋白复合物。由于细胞色素氧化酶是呼吸电子传递链的第四个中心酶复合物，因此又被称为复合物IV。 Picture

Sample:
heart (Mouse) Lysate at 40 ug
Primary: Anti-COX4 (Bs-1533R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 17 kD

Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-COX4/COX ISLV1 Polyclonal Antibody, Unconjugated(SL1533R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Positive control: Hela cells
Concebtration: 5μg/10^6 cells
Incubation conditions: Avoid light , 30 minutes on the ice.

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