Rabbit Anti-AQP9 antibody | Sunlong Medical

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aquaporin Protein-9; Aquaporin 9; AQP 9; AQP9; Aquaporin9; HsT 17287; HsT17287; Small solute channel 1; SSC 1; SSC1; Aquaporin-9; AQP-9; Aquaglyceroporin-9; AQP9_HUMAN.

Cat:

SL2060R

Species Reactivity：

Human,Mouse,(predicted: Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human AQP9:201-295/295<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000Flow-Cyt=1μg /testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:Intestinal(Mouse) lysate at 30 ug Primary: Anti-AQP9 (SL2060R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution; Predicted band size : 32 kDObserved band size : 32 kDSample: Liver (Mouse) Lysate at 40 ugPrimary: Anti-AQP9 (SL2060R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 32 kDObserved band size: 32 kDSample:Liver(Mouse) lysate at 30 ug Primary: Anti-AQP9 (SL2060R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size : 32 kDObserved band size : 32 kDBlank control: A549(blue). Primary Antibody: Rabbit Anti- AQP9/AF647 Conjugated antibody (SL2060R /AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.ProtocolThe cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (SL2060R/AF647, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". A new member of AQP family, Aquaporin-9 (AQP9), has been cloned from human leukocytes by homology cloning (Ishibashi et al. 1997). It is mostly homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295 AA) is primarily expressed in peripheral leukocytes, and lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it did not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and SLCterminus are predicted to be cytoplasmic. A new member of the AQP family, AQP9, has been cloned from human leukocytes. It is homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295aa) is primarily expressed in peripheral leukocytes, and in lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it does not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and SLCterminus are predicted to be cytoplasmic.

Function:
Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na(+), K(+), Cl(-), and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Highly expressed in peripheral leukocytes. Also expressed in liver, lung, and spleen.

Similarity:
Belongs to the MIP/aquaporin (TC 1.A.8) family.

SWISS:
O43315

Gene ID:
366

Database links:

Entrez Gene: 366 Human

Omim: 602914 Human

SwissProt: O43315 Human

Unigene: 104624 Human

br> AQP9是一种选择性水通透膜转运蛋白，属于主体内在蛋白家族的成员之一。AQP9不但对水具有通透性，而且对其它一些中性溶质也具有通透性。 Picture

Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-AQP9 (SL2060R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 32 kD

Sample:Liver(Mouse) lysate at 30 ug
Primary: Anti-AQP9 (SL2060R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 5000 dilution;
Predicted band size : 32 kD
Observed band size : 32 kD

Blank control: A549(blue).
Primary Antibody: Rabbit Anti- AQP9/AF647 Conjugated antibody (SL2060R /AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (SL2060R/AF647, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.

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