Rabbit Anti-GLP-1R antibody

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Glucagon like peptide-1 receptor; GLP 1 R; GLP 1 receptor; GLP 1R; Glp1r; GLP; glp1r; Glucagon like peptide 1 receptor; RATGL1RCP; Glip;MGC138331; GLP-1-R; GLP1R_HUMAN; Glucagon-like peptide 1 receptor; GLP-1 receptor; GLP-1-R; GLP-1R; Precursor.

Cat:

SL1559R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,)

Immunogen：

KLH conjugated synthetic peptide derived from rat GLP-1R:101-200/463<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=2ug/TestICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebrum(Rat) Lysate at 40 ugPrimary: Anti-GLP-1R (SL1559R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 51 kDObserved band size: 51 kDSample: Liver（Mouse） lysate at 30 ug; Primary: Anti- GLP-1R (SL1559R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size: 51 kDObserved band size: 51 kDSample: Lane 1: Pancreas (Mouse) Lysate at 40 ugLane 2: Lung (Mouse) Lysate at 40 ugPrimary: Anti-GLP-1R (SL1559R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 51 kDObserved band size: 51 kDTissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-GLP-1R Polyclonal Antibody, Unconjugated(SL1559R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-GLP-1R Polyclonal Antibody, Unconjugated(SL1559R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control: SH-SY5Y. Primary Antibody (green line): Rabbit Anti-GLP-1R antibody (SL1559R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control: SH-SY5Y. Primary Antibody (green line): Rabbit Anti-GLP-1R antibody (SL1559R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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GLP1R is a receptor for glucagon-like peptide 1. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.It has been suggested that this protein influences the feelings of satiety or hunger, sensation of glucose levels, control of glucagon sensitivity of islets, and non insulin-dependent diabetes mellitus. GLP1R is believed to be expressed in human pancreas,lung, brain, stomach, kidney and heart. ESTs have been isolated from skin and kidney libraries.

Function:
This is a receptor for glucagon-like peptide 1. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

Subunit:
May form homodimers and heterodimers with GIPR.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Post-translational modifications:
N-glycosylation enhances cell surface expression and lengthens receptor half-life by preventing degradation in the ER.

Similarity:
Belongs to the G-protein coupled receptor 2 family.

SWISS:
P32301

Gene ID:
25051

Database links:

Entrez Gene: 2740 Human

Entrez Gene: 14652 Mouse

Entrez Gene: 25051 Rat

Omim: 27632 Human

SwissProt: P43220 Human

SwissProt: O35659 Mouse

SwissProt: P32301 Rat

Unigene: 389103 Human

Unigene: 390969 Mouse

Unigene: 11408 Rat

GLP-1R是GLP-1在体内作用的主要靶点,它是一个7次跨膜的G蛋白偶联受体,以cAMP为主要第二信使。GLP-1与GLP-1R结合过程是一个两结构域相互作用的过程,都与胰岛素分泌和糖代谢调节密切相关。 Picture

Sample: Liver（Mouse） lysate at 30 ug;
Primary: Anti- GLP-1R (SL1559R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000 dilution;
Predicted band size: 51 kD
Observed band size: 51 kD

Sample:
Lane 1: Pancreas (Mouse) Lysate at 40 ug
Lane 2: Lung (Mouse) Lysate at 40 ug
Primary: Anti-GLP-1R (SL1559R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD

Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-GLP-1R Polyclonal Antibody, Unconjugated(SL1559R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-GLP-1R Polyclonal Antibody, Unconjugated(SL1559R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Blank control: SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-GLP-1R antibody (SL1559R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Feedback Wall

Specific References (13) | SL1559R has been referenced in 13 publications.

[IF=3.469] Shuangli Yang. et al. Glucagon-like peptide-1 alleviates diabetic kidney disease through activation of autophagy by regulating AMP-activated protein kinase-mammalian target of rapamycin pathway. Am J Physiol-Endoc M. 2020 Nov;319(6):E1019-E1030 WB,IF ; Rat.

PubMed:32985256

[IF=2.276] Liu Jinlei. et al. Emodin Attenuated the Kidney Damage of High-Fat-Diet Mice via the Upregulation of Glucagon-Like Peptide-1 Receptor. Biomed Res Int. 2021;2021:6662704 WB ; Mouse.

PubMed:34159197

[IF=5.64] Yuping Chen. et al. The Herb Pair Radix Rehmanniae and Cornus Officinalis Attenuated Testicular Damage in Mice With Diabetes Mellitus Through Butyric Acid/Glucagon-Like Peptide-1/Glucagon-Like Peptide-1 Receptor Pathway Mediated by Gut Microbiota. Front Microbiol. 2022 Feb 22;13:831881 IF,WB ; Mouse.

PubMed:35273587

[IF=5.037] Zhan-zhao Liu. et al. Glucagon-like peptide-1 receptor activation by liraglutide promotes breast cancer through NOX4/ROS/VEGF pathway. Life Sci. 2022 Apr;294:120370 WB ; Human.

PubMed:3514800

[IF=3.647] Melek T et al. Ileal interposition improves metabolic syndrome parameters in a rat model of metabolic syndrome induced by monosodium glutamateLife Sci.2020 Dec 9;266:118846. WB ; Rat.

PubMed:33309719

[IF=2.559] Wen Y et al. Inhibitory effects of glucagon-like peptide-1 receptor on epilepsy. Biochem Biophys Res Commun. 2019 Mar 26;511(1):79-86. IP,WB&IHC ; Rat&Human.

PubMed:30770099

[IF=1.448] Bułdak Ł et al. The impact of exenatide (a GLP-1 agonist) on markers of inflammation and oxidative stress in normal human astrocytes subjected to various glycemic conditions. Exp Ther Med. 2019 Apr; 17(4): 2861–2869. WB ; Human.

PubMed:30906473

[IF=3.288] Kanda et al. Expression of the glucagon-like peptide-1 receptor and its role in regulating autophagy in endometrial cancer. (2018) BMC.Cancer. 18:657 WB ; Human.

PubMed:29907137

[IF=5.682] Haoqiang Zhang. et al. Glucagon-like peptide-1 attenuated carboxymethyl lysine induced neuronal apoptosis via peroxisome proliferation activated receptor-γ. Aging-Us. 2021 Jul 31; 13(14): 19013–19027 WB ; Rat.

PubMed:34326274

[IF=5.7] Shan Y et al. The glucagon-like peptide-1 receptor agonist reduces inflammation and blood-brain barrier breakdown in an astrocyte-dependent manner in experimental stroke. J Neuroinflammation. 2019 Nov 28;16(1):242. IHF ; Rat.

PubMed:31779652

[IF=2.395] Kim SJ et al. DPP-4 inhibition enhanced renal tubular and myocardial GLP-1 receptor expression decreased in CKD with myocardial infarction.BMC Nephrol. 2019 Mar 1;20(1):75. WB ; Rat.

PubMed:30823876

[IF=3.06] Nagai, Takuo, et al. "Linagliptin Ameliorates Methylglyoxal-Induced Peritoneal Fibrosis in Mice." PLOS ONE 11.8 (2016): e0160993. WB, IHSLCP ; Mouse.

PubMed:27513960

[IF=5.71] Zhou, Hao, et al. "Exendin-4 protects ADMSCs from apoptosis induced by Hydrogen peroxide through PI3K/Akt-Sfrp2 pathways." Free Radical Biology and Medicine (2014). Rat.

PubMed:25452142