Rabbit Anti-Dopamine D3 receptor antibody

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DRD3; 3dopamine receptor; D; Dopamine receptor D3; DRD3; Dopamine receptor 3; ETM1; FET1; D(3) dopamine receptor; DRD3; DRD3_HUMAN.

Cat:

SL1743R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from mouse DRD3:352-446/446<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:200-800（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Brain (Mouse) Lysate at 40 ugBrain (Rat) Lysate at 40 ugPrimary: Anti-Dopamine D3 receptor (SL1743R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 44 kDObserved band size: 48 kDSample: HepG2 Cell Lysate at 30 ugU251 Cell Lysate at 30 ugPrimary: Anti-Dopamine D3 receptor (SL1743R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 44 kDObserved band size: 48 kDParaformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dopamine D3 receptor) Polyclonal Antibody, Unconjugated (SL1743R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dopamine D3 receptor) Polyclonal Antibody, Unconjugated (SL1743R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF594) for 90 minutes, and DAPI for nuclei staining.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. This receptor is expressed in phylogenetically older regions of the brain, suggesting that it plays a role in cognitive and emotional functions. It is a target for drugs which treat schizophrenia, drug addiction, and Parkinson disease. Alternative splicing of this gene results in multiple transcript variants that would encode different isoforms, although some variants may be subject to nonsense-mediated decay (NMD). Also known as: dopamine D3 receptor; 3 dopamine receptor; D; Dopamine receptor D3; DRD; ETM1; FET1.

Subunit:
Interacts with CLIC6. Interacts with GRK4. Interacts with PALM.

Subcellular Location:
Cell membrane; Multi-pass membrane protein. Note=Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells.

Tissue Specificity:
Brain.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P30728

Gene ID:
13490

Database links:

Entrez Gene: 1814 Human

Entrez Gene: 13490 Mouse

Entrez Gene: 29238 Rat

Omim: 126451 Human

SwissProt: P35462 Human

SwissProt: P30728 Mouse

SwissProt: P19020 Rat

Unigene: 121478 Human

Unigene: 439735 Mouse

Unigene: 10356 Rat

多巴胺受体D3常与某些精神、神经疾病有关，如精神分裂症、早发性痴呆等。但最新研究表明多巴胺受体与肿瘤细胞多药耐药有关。 Picture

Sample:
HepG2 Cell Lysate at 30 ug
U251 Cell Lysate at 30 ug
Primary: Anti-Dopamine D3 receptor (SL1743R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 48 kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dopamine D3 receptor) Polyclonal Antibody, Unconjugated (SL1743R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dopamine D3 receptor) Polyclonal Antibody, Unconjugated (SL1743R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF594) for 90 minutes, and DAPI for nuclei staining.

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