Rabbit Anti-IL12 antibody | Sunlong Medical

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Interleukin-12 subunit alpha; IL-12; CLMF p35; CLMF1; CTL maturation factor (TcMF); Cytotoxic lymphocyte maturation factor 1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL 12 subunit p35; IL12A; Interleukin 12 alpha chain; Interleukin 12 p35;

Cat:

SL1789R

Species Reactivity：

Human,

Immunogen：

KLH conjugated synthetic peptide derived from human IL-12:51-150/219

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: U251 Cell Lysate at 30 ugPrimary: Anti- IL12 (SL1789R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 23 kDObserved band size: 34 kDSample: MDA-MB-231(Human) Cell Lysate at 40 ugPrimary: Anti-IL12 (SL1789R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 23 kDObserved band size: 34 kDTissue/cell: human colon carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min; Incubation: Anti-IL12 Polyclonal Antibody, Unconjugated(SL1789R) 1:500, overnight at 4∑C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37∑C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nucleiTissue/cell: human lung carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min; Incubation: Anti-IL12 Polyclonal Antibody, Unconjugated(SL1789R) 1:500, overnight at 4∑C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37∑C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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IL-12 protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

Function:
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Subunit:
Heterodimer with IL12B; disulfide-linked. The heterodimer is known as interleukin IL-12.

Subcellular Location:
Secreted.

Similarity:
Belongs to the IL-6 superfamily.

SWISS:
P29459

Gene ID:
3592

Database links:

Entrez Gene: 3592 Human

Entrez Gene: 3593 Human

Omim 161560 Human

Omim: 161561 Human

SwissProt: P29459 Human

SwissProt: P2992 Human

Unigene: 673 Human

Unigene: 674 Human

IL-12是新近发现的细胞因子，具有多种生物学活性，尤其是在抗肿瘤免疫和抗病毒免疫中有重要的作用。 Picture

Sample: U251 Cell Lysate at 30 ug
Primary: Anti- IL12 (SL1789R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 34 kD

Sample:
MDA-MB-231(Human) Cell Lysate at 40 ug
Primary: Anti-IL12 (SL1789R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 34 kD

Tissue/cell: human colon carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min;
Incubation: Anti-IL12 Polyclonal Antibody, Unconjugated(SL1789R) 1:500, overnight at 4∑C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37∑C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

Tissue/cell: human lung carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min;
Incubation: Anti-IL12 Polyclonal Antibody, Unconjugated(SL1789R) 1:500, overnight at 4∑C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37∑C. DAPI(5ug/ml,blue,SLC0033) was used to stain the cell nuclei

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