Rabbit Anti-PPARGC1A antibody

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LEM6; PGC-1 Alpha; PGC1 Alpha; Ligand effect modulator 6; Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha; Peroxisome proliferative activated receptor, gamma, coactivator 1 ; Peroxisome proliferator activated receptor gamma coactiv

Cat:

SL1832R

Species Reactivity：

Human,Mouse,(predicted: Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human PGC-1:601-700/798

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Thymus (Mouse) Lysate at 40 ugPrimary: Anti-PPARGC1A (SL1832R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 88 kDObserved band size: 88 kDSample: Heart (Mouse) Lysate at 40 ugPrimary: Anti-PPARGC1A (SL1832R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 88 kDObserved band size: 90 kDSample: Heart (Mouse) Lysate at 30 ugPrimary: Anti- PPARGC1A (SL1832R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilutionPredicted band size: 88 kDObserved band size: 88 kDTissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-PPARGC1A/PGC1 alpha Polyclonal Antibody, Unconjugated(SL1832R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control: HepG2. Primary Antibody (green line): Rabbit Anti-PPARGC1A antibody (SL1832R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647Dilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity (referenced from entrez gene.

Function:
Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism.

Subunit:
Binds MYBBP1A, which inhibits transcriptional activation by this protein. Interacts with PRDM16. Interacts with LRPPRC. Homooligomer. Interacts with LPIN1.

Subcellular Location:
Nucleus.

Tissue Specificity:
Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects.

Post-translational modifications:
hosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2.
Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.

Similarity:
Contains 1 RRM (RNA recognition motif) domain.

SWISS:
Q9UBK2

Gene ID:
10891

Database links:

Entrez Gene: 422815 Chicken

Entrez Gene: 338446 Cow

Entrez Gene: 479127 Dog

Entrez Gene: 10891 Human

Entrez Gene: 19017 Mouse

Entrez Gene: 497232 Pig

Entrez Gene: 83516 Rat

Omim: 604517 Human

SwissProt: Q865B7 Cow

SwissProt: Q9UBK2 Human

SwissProt: O70343 Mouse

SwissProt: Q865B6 Pig

SwissProt: Q9QYK2 Rat

Unigene: 527078 Human

Unigene: 259072 Mouse

Unigene: 19172 Rat

PGSLC1α是一个新发现的转录激活因子,起到高游离脂肪酸刺激胰岛α细胞分泌的重要调控作用。 Picture

Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti-PPARGC1A (SL1832R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kD
Observed band size: 90 kD

Sample: Heart (Mouse) Lysate at 30 ug
Primary: Anti- PPARGC1A (SL1832R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 88 kD
Observed band size: 88 kD

Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-PPARGC1A/PGC1 alpha Polyclonal Antibody, Unconjugated(SL1832R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-PPARGC1A antibody (SL1832R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Specific References (25) | SL1832R has been referenced in 25 publications.

[IF=14.528] IF ; mouse.

PubMed:32761924

[IF=3.512] Piao Wang. et al. Effects of Root Extract of Morinda officinalis in Mice with High-Fat-Diet/Streptozotocin-Induced Diabetes and C2C12 Myoblast Differentiation. Acs Omega. 2021;6(41):26959–26968 WB ; Mouse.

PubMed:34693116

[IF=5.455] Li, Xuling. et al. Intake of flavonoids from Astragalus membranaceus ameliorated brain impairment in diabetic mice via modulating brain-gut axis. Chin Med-Uk. 2022 Dec;17(1):1-19 WB,IF ; Mouse.

PubMed:35151348

[IF=5.209] Xianglun Zhang. et al. Protective effect of resveratrol against hydrogen peroxide-induced oxidative stress in bovine skeletal muscle cells. Meat Sci. 2022 Mar;185:108724 WB ; Cattle.

PubMed:34952489

[IF=6.048] He Zhang. et al. Feeding frequency affects glucose and lipid metabolism through SIRT1/AMPK pathway in growing pigs with the same amount of daily feed. J Nutr Biochem. 2021 Nov;:108919 WB ; Pig.

PubMed:34843934

[IF=3.998] Renli Qi. et al. The intestinal microbiota contributes to the growth and physiological state of muscle tissue in piglets. Sci Rep-Uk. 2021 May;11(1):1-14 WB ; Pig.

PubMed:34045661

[IF=3.046] Jia Wang. et al. Ameliorative effect of SIRT1 in postpartum depression mediated by upregulation of the glucocorticoid receptor. Neurosci Lett. 2021 Sep;761:136112 WB ; Rat.

PubMed:34265417

[IF=2.639] Biqi Han. et al. Harmful Effects of Inorganic Mercury Exposure on Kidney Cells: Mitochondrial Dynamics Disorder and Excessive Oxidative Stress. 2021 May 31 WB ; Human.

PubMed:34060062

[IF=1.662] Jianbin Xue. et al. The Sirtuin‐1 relied antioxidant and antiaging activity of 5,5′‐diferulic acid glucoside esters derived from corn bran by enzymatic method. J Food Biochem. 2020 Dec;44(12):e13519 WB ; Human.

PubMed:33078415

[IF=2.678] Jingsong Xiao. et al. PGSLC1ɑ Mediated-EXOG, a Specific Repair Enzyme for Mitochondrial DNA, Plays an Essential Role in the Rotenone-Induced Neurotoxicity of PC12 Cells. 2021 Jan 30 WB ; Rat.

PubMed:33515431

[IF=6.792] Yang Q et al. Hexavalent chromium induces mitochondrial dynamics disorder in rat liver by inhibiting AMPK/PGSLC1α signaling pathway. Environ Pollut.2020 Oct;265(Pt A):114855. WB ; Rat.

PubMed:32474337

[IF=3.448] Dai Y et al. Inhibition of miR‐23a‐3p promotes osteoblast proliferation and differentiation. J Cell Biochem. 2019 Nov 6. IHSLCP&WB ; Rat.

PubMed:31691358

[IF=3.974] Ning X et al.Ambient PM2. 5 causes lung injuries and coupled energy metabolic disorder. (2018) Ecotoxicol. Environ. Saf. 170 WB ; Mouse.

PubMed:30579162

[IF=2.09] Lu, Jun-Juan, et al. "Tumor necrosis factor-like weak inducer of apoptosis regulates quadriceps muscle atrophy and fiber-type alteration in a rat model of chronic obstructive pulmonary disease." Tobacco Induced Diseases 15.1 (2017): 43. WB ; Rat.

PubMed:29151827

[IF=1.35] Hui, Yan, et al. "Resveratrol improves mitochondrial function in the remnant kidney from 5/6 nephrectomized rats." Acta Histochemica (2017). WB ; Rat.

PubMed:28434671

[IF=6.529] Nan Chen. et al. β2-adrenoreceptor agonist ameliorates mechanical allodynia in paclitaxel-induced neuropathic pain via induction of mitochondrial biogenesis. Biomed Pharmacother. 2021 Dec;144:112331 IF ; Rar.

PubMed:34673421

[IF=5.858] Yue Ding. et al. Protective role of sirtuin3 against oxidative stress and NLRP3 inflammasome in cholesterol accumulation and foam cell formation of macrophages with ox-LDL-stimulation. Biochem Pharmacol. 2021 Oct;192:114665 WB ; Mouse.

PubMed:34181898

[IF=3.647] Renli Qi. et al. MicroRNA-489-3p promotes adipogenesis by targeting the Postn gene in 3T3-L1 preadipocytes. Life Sci. 2021 Aug;278:119620 WB ; Mouse.

PubMed:34004251

[IF=14.528] Dejun Xu. et al. Melatonin protects mouse testes from palmitic acid‐induced lipotoxicity by attenuating oxidative stress and DNA damage in a SIRT1‐dependent manner. J Pineal Res. 2020 Nov;69(4):e12690 WB ; Mouse.

PubMed:32761924

[IF=3.191] Jing Luo. et al. Cardiac Troponin I R193H Mutation Is Associated with Mitochondrial Damage in Cardiomyocytes. Dna Cell Biol. 2021 Feb;40(2):184-191 WB ; Mouse.

PubMed:33465007

[IF=4.02] Qin, Guohua, et al. "Sulfur dioxide inhalation stimulated mitochondrial biogenesis in rat brains." Toxicology 300.1 (2012): 67-74. WB ; Rat.

PubMed:22677886

[IF=2.078] Shi L et al. β1 adrenoceptor antibodies induce myocardial apoptosis via inhibiting PGSLC1α-related pathway. BMC Cardiovasc Disord. 2020 Jun 5;20(1):269. WB ; Rat.

PubMed:32503464

[IF=1.358] Ma WF et al. Vanillic acid alleviates palmitic acid‐induced oxidative stress in human umbilical vein endothelial cells via Adenosine Monophosphate‐Activated Protein Kinase signaling pathway. Journal of Food Biochemistry,2019 e12893. WB ; Human.

PubMed:doi:10.1111/jfbc.12893

[IF=13.03] Chen, Yue, et al. "Nanosilver Incurs an Adaptive Shunt of Energy Metabolism Mode to Glycolysis in Tumor and Non-Tumor Cells." ACS Nano (2014). WB ; Human.

PubMed:24810997

[IF=3.159] Wan Boyang. et al. Zearalenone promotes follicle development through activating SIRT1/PGSLC1α signaling pathway in the ovaries of weaned gilts. J Anim Sci. 2022 Feb;: WB,IHC ; Pig(Gilt).

PubMed:10.1093/jas/skac058