Rabbit Anti-Aldolase A antibody

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ALDA; Aldo1; ALDOA; ALDOA_HUMAN; Aldolase 1; Aldolase A; Aldolase A fructose bisphosphatase; Aldolase A fructose bisphosphate; FRUCTOALDOLASE A; Fructose 1 6 bisphosphate triosephosphate lyase; Fructose bisphosphate aldolase A; Fructose bisphos

Cat:

SL486R

Species Reactivity：

Mouse,(predicted: Human,Rat,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human Aldolase A:261-364/364

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg /testIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Muscle (Mouse) Lysate at 40 ugPrimary: Anti-Aldolase A (SL486R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 39 kDObserved band size: 39 kDParaformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Aldolase A) Polyclonal Antibody, Unconjugated (SL486R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control(blue): Mouse spleen(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti-UCP-1 antibody(SL1925R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA. Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ). Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Research areas:Cancer //Cancer Metabolism //Metabolic signaling pathway <
Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.
Cellular localization:Cytoplasmic
Tissue Specificity:adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue.

Function:
Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.

Subunit:
Homotetramer. Interacts with SNX9 and WAS.

DISEASE:
Glycogen storage disease 12 (GSD12) [MIM:611881]: A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the class I fructose-bisphosphate aldolase family.

SWISS:
P04075

Gene ID:
226

Database links:

Entrez Gene: 226 Human

Omim: 103850 Human

SwissProt: P04075 Human

Unigene: 513490 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Aldolase A) Polyclonal Antibody, Unconjugated (SL486R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Blank control(blue): Mouse spleen(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody:Rabbit Anti-UCP-1 antibody(SL1925R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA.
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ).
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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