Rabbit Anti-GPNMB antibody | Sunlong Medical

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NMB; Glycoprotein nmb like protein; Gpnmb; DC-HIL; Dchil; ipd; Glycoprotein (transmembrane) nmb; Glycoprotein nmb; HGFIN; Transmembrane glycoprotein; Transmembrane glycoprotein HGFIN; Transmembrane glycoprotein NMB; Osteoactivin; GPNMB_HUMAN.

Cat:

SL2684R

Species Reactivity：

Human,(predicted: Pig,Cow,Horse,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from human GPNMB:81-180/572<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: PANSLC1(Human) Cell Lysate at 30 ugPrimary: Anti-GPNMB (SL2684R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 61 kDObserved band size: 101 kDTissue/cell: rat lung tissue(melanoma); 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37鈩� for 20 min; Incubation: Anti-GPNMB/Osteoactivin Polyclonal Antibody, Unconjugated(SL2684R) 1:200, overnight at 4掳C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
Could be a melanogenic enzyme.

Subcellular Location:
Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Tissue Specificity:
Not restricted to the melanocytic lineage.

Similarity:
Belongs to the PMEL/NMB family.
Contains 1 PKD domain.

SWISS:
Q14956

Gene ID:
10457

Database links:

Entrez Gene: 10457 Human

Entrez Gene: 93695 Mouse

Entrez Gene: 113955 Rat

Omim: 604368 Human

SwissProt: Q14956 Human

SwissProt: Q99P91 Mouse

SwissProt: Q6P7C7 Rat

Unigene: 190495 Human

Unigene: 302602 Mouse

Unigene: 13778 Rat

Picture

Tissue/cell: rat lung tissue(melanoma); 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37鈩� for 20 min;
Incubation: Anti-GPNMB/Osteoactivin Polyclonal Antibody, Unconjugated(SL2684R) 1:200, overnight at 4掳C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Specific References (8) | SL2684R has been referenced in 8 publications.

[IF=0] Sondag, Gregory R. OSTEOACTIVIN AND CD44: A NOVEL INTERACTION REGULATING BONE CELL DIFFERENTIATION AND FUNCTION. Diss. Kent State University, 2015. WB ; Mouse.

PubMed:Diss.KentStateUniversity,2015.

[IF=5.1] Neal et al. The glycoprotein GPNMB attenuates astrocyte inflammatory responses through the CD44 receptor. (2018) J.Neuroinflammation. 15:73 IF(ICC) ; Mouse.

PubMed:29519253

[IF=1.42] Ruan, Wei-Min, et al. "Differential expression of glycoprotein non-metastatic melanoma protein B (GPNMB) involved in trichostatin A-induced apoptosis in gastric cancer." International journal of clinical and experimental medicine 7.12 (2014): 4857-4866. IHSLCP ; Human.

PubMed:25663982

[IF=4.183] Zhu Y et al. Protein Expression Profile in Rat Silicosis Model Reveals Upregulation of PTPN2 and Its Inhibitory Effect on Epithelial-Mesenchymal Transition by Dephosphorylation of STAT3. Int J Mol Sci. 2020 Feb 11;21(4). WB ; Rat&Mouse.

PubMed:6454021

[IF=5.17] Oyewumi et al. Osteoactivin (GPNMB) ectodomain protein promotes growth and invasive behavior of human lung cancer cells. (2016) Oncotarge. 7:13932-44 IHC ; Human.

PubMed:26883195

[IF=1.72] Frara, Nagat, et al. "Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping." BMC Musculoskeletal Disorders 17.1 (2016): 1. WB ; Rat.

PubMed:26781168

[IF=3.84] Arosarena, Oneida A., et al. "Osteoactivin Promotes Migration of Oral Squamous Cell Carcinomas." Journal of Cellular Physiology (2016). WB ; Human.

PubMed:26636434

[IF=4.6] Abdelmagid, Samir M., et al. "Mutation in Osteoactivin Promotes RANKL-Mediated Osteoclast Differentiation and Survival, but Inhibits Osteoclast Function." Journal of Biological Chemistry (2015): jbc-M114. WB ; Mouse.

PubMed:25837253