Rabbit Anti-phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) antibody

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GSK3B(Phospho-Tyr279+Tyr216); GSK3B(Phospho-Y279/Y216); p-GSK-3 Beta(Tyr279+Tyr216); p-GSK-3 beta(Y279/Y216); Glycogen synthase kinase 3 beta; GSK 3 beta; GSK 3B; GSK3B; GSK3B protein; GSK3beta isoform; GSK3 beta; Glycogen synthase kinase-3 beta; GSK-3 be

Cat:

SL2073R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,Guinea Pig,)

Immunogen：

KLH conjugated Synthesised phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Tyr216:VS(p-Y)IC

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg /testIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Muscle (Mouse) Lysate at 40 ugLane 2: Liver (Mouse) Lysate at 40 ugLane 3: Cerebrum (Mouse) Lysate at 40 ugLane 4: Pancreas (Mouse) Lysate at 40 ugLane 5: Thymus (Mouse) Lysate at 40 ugLane 6: Muscle (Rat) Lysate at 40 ugLane 7: Liver (Rat) Lysate at 40 ugLane 8: Cerebrum (Rat) Lysate at 40 ugLane 9: Pancreas (Rat) Lysate at 40 ugLane 10: Thymus (Rat) Lysate at 40 ugLane 11: MCF-7 (Human) Cell Lysate at 30 ugLane 12: A431 (Human) Cell Lysate at 30 ugLane 13: U2os (Human) Cell Lysate at 30 ugPrimary: Anti-phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) (SL2073R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 47’51 kDObserved band size: 47 kDSample: brain (Mouse) Lysate at 40 ugPrimary:Anti-phospho-GSK3Alpha+Beta(Tyr279+Tyr216)(SL2073R)at1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 47/51 kDObserved band size: 47 kDProtein:Heart(Mouse) lysate at 30ug; Primary: Anti-phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) (SL2073R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size: 47/51 kDObserved band size: 47 kDSample: Lane1: Brain (Mouse) Lysate at 30 ugLane2: Testis(Mouse) Lysate at 30 ugPrimary: Anti-phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) (SL2073R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size:47/51 kDObserved band size:50 kDPositive control: RSC96 Isotype Control Antibody: Rabbit IgG; Secondary Antibody: Goat anti-rabbit IgG-FITC;Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody catalog number: SL2073R; Dilution: 3μL in 100 μl 1X PBS containing 0.5% BSA

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.

Function:
Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates NFATC1/NFATC on conserved serine residues promoting NFATC1/NFATC nuclear export, shutting off NFATC1/NFATC gene regulation, and thereby opposing the action of calcineurin. Phosphorylates MAPT/TAU on 'Thr-548', decreasing significantly MAPT/TAU ability to bind and stabilize microtubules. MAPT/TAU is the principal component of neurofibrillary tangles in Alzheimer disease. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Phosphorylates MACF1, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. Probably regulates NF-kappa-B (NFKB1) at the transcriptional level and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF-alpha (TNF/TNFA). Negatively regulates replication in pancreatic beta-cells, resulting in apoptosis, loss of beta-cells and diabetes. Phosphorylates MUC1 in breast cancer cells, decreasing the interaction of MUC1 with CTNNB1/beta-catenin. Is necessary for the establishment of neuronal polarity and axon outgrowth. Phosphorylates MARK2, leading to inhibit its activity. Phosphorylates SIK1 at 'Thr-182', leading to sustain its activity.

Subunit:
Monomer. Interacts with ARRB2 and DISC1. Interacts with CABYR, MMP2, MUC1, NIN and PRUNE Interacts with AXIN1; the interaction mediates hyperphosphorylation of CTNNB1 leading to its ubiquitination and destruction. Interacts with and phosphorylates SNAI1. Interacts with DNM1L (via a SLCterminal domain). Found in a complex composed of MACF1, APC, AXIN1, CTNNB1 and GSK3B.

Subcellular Location:
Cytoplasm. Nucleus. Cell membrane. Note=The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.

Tissue Specificity:
Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.

Post-translational modifications:
Phosphorylated by AKT1 and ILK1. Upon insulin-mediated signaling, the activated PKB/AKT1 protein kinase phosphorylates and desactivates GSK3B, resulting in the dephosphorylation and activation of GYS1. Activated by phosphorylation at Tyr-216.

Similarity:
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain.

SWISS:
P49841

Gene ID:
2932

Database links:

Entrez Gene: 2932 Human

Entrez Gene: 56637 Mouse

Omim: 605004 Human

SwissProt: P49841 Human

SwissProt: Q9WV60 Mouse

Unigene: 445733 Human

Unigene: 394930 Mouse

Picture

Sample:
brain (Mouse) Lysate at 40 ug
Primary:Anti-phospho-GSK3Alpha+Beta(Tyr279+Tyr216)(SL2073R)at1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47/51 kD
Observed band size: 47 kD

Protein:Heart(Mouse) lysate at 30ug;
Primary: Anti-phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) (SL2073R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000 dilution;
Predicted band size: 47/51 kD
Observed band size: 47 kD

Sample:
Lane1: Brain (Mouse) Lysate at 30 ug
Lane2: Testis(Mouse) Lysate at 30 ug
Primary: Anti-phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) (SL2073R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000 dilution;
Predicted band size:47/51 kD
Observed band size:50 kD

Positive control: RSC96
Isotype Control Antibody: Rabbit IgG; Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody catalog number: SL2073R; Dilution: 3μL in 100 μl 1X PBS containing 0.5% BSA

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