Rabbit Anti-CXCR3 antibody | Sunlong Medical

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CXCR3_HUMAN; Interferon-inducible protein 10 receptor; IP-10 receptor; C-X-C chemokine receptor type 3; CD 183; CD183; CD183 antigen; G protein-coupled receptor 9; G Protein Coupled Receptor 9; Chemokine (C X C motif) receptor 3; Chemokine (C X C) recepto

Cat:

SL2209R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Rabbit,Guinea Pig,)

Immunogen：

KLH conjugated synthetic peptide derived from mouse CXCR3:301-367/367

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000Flow-Cyt=0.2μg /testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Mouse Blood cell tissue lysates Lane 2: Rat Blood cell tissue lysatesPrimary: Anti-CXCR3 (SL2209R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 40 kDaObserved band size: 40 kDaBlank control: U937. Primary Antibody (green line): Rabbit Anti-CXCR3 antibody (SL2209R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control: HCCLM3(blue)Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5ul in 100 uL1X PBS containing 0.5% BSA(green).

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716). [provided by RefSeq, Jun 2011].

Function:
Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC).
Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). Interaction with CXCL4 or CXCL10 leads to activation of the p38MAPK pathway and contributes to inhibition of angiogenesis. Overexpression in renal cancer cells down-regulates expression of the anti-apoptotic protein HMOX1 and promotes apoptosis.
Isoform 3 mediates the activity of CXCL11.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta.

Post-translational modifications:
Sulfation on Tyr-27 and Tyr-29 is essential for CXCL10 binding and subsequent signal transduction induction.
N-glycosylated.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
O88410

Gene ID:
12766

Database links:

Entrez Gene: 12766 Mouse

SwissProt: O88410 Mouse

Unigene: 12876 Mouse

Picture

Blank control: U937.
Primary Antibody (green line): Rabbit Anti-CXCR3 antibody (SL2209R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Blank control: HCCLM3(blue)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5ul in 100 uL1X PBS containing 0.5% BSA(green).

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Specific References (11) | SL2209R has been referenced in 11 publications.

[IF=13.751] Allison A Fitzgerald. et al. DPP inhibition alters the CXCR3 axis and enhances NK and CD8+ T cell infiltration to improve anti-PD1 efficacy in murine models of pancreatic ductal adenocarcinoma. J Immunother Cancer. 2021 Nov;9(11):e002837 IHC ; Mouse.

PubMed:34737215

[IF=4.044] Malte Puchert. et al. Identification of CXCL11 as part of chemokine network controlling skeletal muscle development. 2021 Jan 27 WB,IF,IHC ; Rat.

PubMed:33502606

[IF=3.078] Puchert M et al. CXCL11 promotes tumor progression by the biased use of the chemokine receptors CXCR3 and CXCR7. Cytokine. 2019 Aug 19;125:15969. WB ; Human.

PubMed:31437604

[IF=0] Deyhle et al. CXCL10 increases in human skeletal muscle following damage but is not necessary for muscle regeneration. (2018) Physiol.Rep. 6:e13689 IHC ; mouse.

PubMed:29696819

[IF=4.88] Massafra, Vittoria, et al. "Splenic dendritic cell involvement in FXR-mediated amelioration of DSS colitis." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease (2015). IHSLCP ; Mouse.

PubMed:2655925

[IF=4.2] Ge, L. P., et al. "Integration of nondegradable polystyrene and degradable gelatin in a core–sheath nanofibrous patch for pelvic reconstruction." International Journal of Nanomedicine 2015:10 3193–641 IHSLCP ; Rat.

PubMed:25995629

[IF=2.784] Yin M et al. Protective effects of CXCR3/HO‑1 gene‑modified BMMSCs on damaged intestinal epithelial cells: Role of the p38‑MAPK signaling pathway.Int J Mol Med. 2019 May;43(5):2086-2102. ICC ; Mouse.

PubMed:30864136

[IF=5.51] Baur et al. The Transcription Factor NFATc1 Supports the Rejection of Heterotopic Heart Allografts. (2018) Front.Immunol. 9:1338 IHC ; Mouse.

PubMed:29946322

[IF=2] Wang, Jun, et al. "Anti‐inflammatory and retinal protective effects of capsaicin on ischemia‐induced injuries through the release of endogenous somatostatin." Clinical and Experimental Pharmacology and Physiology (2017). IHSLCP ; Mouse.

PubMed:28429852

[IF=3.06] Zhang, Wen, et al. "The role of CXCR3 in the induction of primary biliary cirrhosis." Clinical and Developmental Immunology 2011 (2011). IHSLCP ; Mouse.

PubMed:21647407

[IF=5.94] Saxena, Amit, et al. "CXCR3-independent actions of the CXC chemokine CXCL10 in the infarcted myocardium and in isolated cardiac fibroblasts are mediated through proteoglycans." Cardiovascular Research (2014): cvu138. Mouse.

PubMed:24891401