Rabbit Anti-Phospho-FRA1 (Ser265) antibody

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FOSL1; Fos related antigen 1; FOS L1; FOS like antigen 1; FOSL 1; FOSL1 protein; FRA 1; Fos-related antigen 1; FOSL1_HUMAN; FRA-1.

Cat:

SL3158R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated Synthesised phosphopeptide derived from human FRA1 around the phosphorylation site of Ser265:LG(p-S)PT

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Hela(Human) Cell Lysate at 30 ugU87MG(Human) Cell Lysate at 30 ugPrimary: Anti- Phospho-FRA1 (Ser265) (SL3158R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 28 kDObserved band size: 28 kDParaformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-FRA1 (Ser265)) Polyclonal Antibody, Unconjugated (SL3158R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-Phospho-FRA1(Ser265) Polyclonal Antibody, Unconjugated(SL3158R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. [provided by RefSeq].

Subunit:
Heterodimer.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP (basic-leucine zipper) domain.

SWISS:
P15407

Gene ID:
8061

Database links:

Entrez Gene: 8061 Human

Omim: 136515 Human

SwissProt: P15407 Human

Unigene: 283565 Human

FOSL1蛋白对细胞因子、生长因子、感染或致癌刺激等生理或病理信号发生应答,调节基因的转录, 参与细胞的增殖、分化等过程。 FOSL1在肿瘤形成及发展过程中, 通过促进细胞增殖、抑制分化、促进肿瘤细胞的侵袭和转移等过程发挥作用。 Picture

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-FRA1 (Ser265)) Polyclonal Antibody, Unconjugated (SL3158R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-FRA1(Ser265) Polyclonal Antibody, Unconjugated(SL3158R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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