Rabbit Anti-FCGR1A/CD64 antibody

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High affinity immunoglobulin gamma Fc receptor I; CD 64; CD64 antigen; CD64A; Fc fragment of IgG high affinity Ia receptor; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for; Fc fragment of IgG, high

Cat:

SL3511R

Species Reactivity：

Human,Mouse,Rat,(predicted: Pig,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human CD64/IGFR1:101-200/374<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000Flow-Cyt=1μg /testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Mouse Spleen tissue lysates Lane 2: Mouse Lung tissue lysates Lane 3: Mouse Liver tissue lysates Lane 4: Mouse Kidney tissue lysates Lane 5: Mouse Raw264.7 cell lysates Lane 6: Rat Spleen tissue lysates Lane 7: Rat Liver tissue lysates Lane 8: Rat Kidney tissue lysates Lane 9: Human THP-1 cell lysates Lane 10: Human HL60 cell lysates Primary: Anti- FCGR1A/CD64 (SL3511R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 41 kDaObserved band size: 70 kDaBlank control: Mouse spleen cells(blue). Primary Antibody: Rabbit Anti- FCGR1A/CD64 antibody(SL3511R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.ProtocolPrimary antibody (SL3511R, 5μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.Blank control: 293T Cells(blue). Primary Antibody: Rabbit Anti-FCGR1A/CD64/AF647 Conjugated antibody (SL3511R-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD64 antibody(SL3511R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]

Subunit:
Interacts with FCERG1; forms a functional signaling complex. Interacts with FLNA; prevents FCGR1A degradation. Interacts with EPB41L2, LAT and PPL. Interacts with HCK and LYN.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Note=Stabilized at the cell membrane through interaction with FCER1G.

Tissue Specificity:
Monocyte/macrophage specific.

Similarity:
Belongs to the immunoglobulin superfamily. FCGR1 family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.

SWISS:
P12314

Gene ID:
2209

Database links:

Entrez Gene: 2209 Human

Omim: 146760 Human

SwissProt: P12314 Human

Unigene: 77424 Human

CD64是一种糖蛋白，主要定位于单核细胞和巨噬细胞,参与免疫应答反应。 Picture

Blank control: Mouse spleen cells(blue).
Primary Antibody: Rabbit Anti- FCGR1A/CD64 antibody(SL3511R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
Primary antibody (SL3511R, 5μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

Blank control: 293T Cells(blue).
Primary Antibody: Rabbit Anti-FCGR1A/CD64/AF647 Conjugated antibody (SL3511R-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.

Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody: Rabbit Anti-CD64 antibody(SL3511R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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