Rabbit Anti-phospho-KLF5 (Ser311) antibody

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KLF5 (phospho Ser311); KLF5 (phospho S311); Kinesin 5B; Klf5; CKLF; Krueppel-like factor 5; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2; Basic transcription element-binding protein 2; BTE-binding protein 2; Krueppel-like factor 5;

Cat:

SL2303R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthesised phosphopeptide derived from human KLF5 around the phosphorylation site of Ser311:PG(p-S)PD

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-KLF5(Ser311)) Polyclonal Antibody, Unconjugated (SL2303R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-KLF5(Ser311)) Polyclonal Antibody, Unconjugated (SL2303R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-KLF5(Ser311)) Polyclonal Antibody, Unconjugated (SL2303R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat intestine tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLF5(Ser311)) Polyclonal Antibody, Unconjugated (SL2303R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Kinesin 5B; Klf5; CKLF; Krueppel-like factor 5; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2; Basic transcription element-binding protein 2; BTE-binding protein 2; Krueppel-like factor 5; KLF5; basic transcription element-binding protein 2;BTEB2; intestine enriched krüppel-like factor; IKLF; Kruppel like factor 5.

Function:
Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.

Subunit:
Interacts with WWP1.

Subcellular Location:
Nucleus.

Tissue Specificity:
Expressed only in testis and placenta.

Post-translational modifications:
Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.

Similarity:
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.

SWISS:
Q13887

Gene ID:
688

Database links:

Entrez Gene: 535702 Cow

Entrez Gene: 688 Human

Entrez Gene: 12224 Mouse

Entrez Gene: 100038005 Pig

Entrez Gene: 84410 Rat

Omim: 602903 Human

SwissProt: Q13887 Human

SwissProt: Q9Z0Z7 Mouse

Unigene: 508234 Human

Unigene: 30262 Mouse

Unigene: 8954 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-KLF5(Ser311)) Polyclonal Antibody, Unconjugated (SL2303R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-KLF5(Ser311)) Polyclonal Antibody, Unconjugated (SL2303R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat intestine tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLF5(Ser311)) Polyclonal Antibody, Unconjugated (SL2303R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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