This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
Subunit:
Homodimer.
Post-translational modifications:
The initiator methionine for isoform 2 is removed.
DISEASE:
Triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]: Autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Note=The disease is caused by mutations affecting the gene represented in this entry.
Similarity:
Belongs to the triosephosphate isomerase family.
SWISS:
P60174
Gene ID:
7167
Database links:
UniProtKB/Swiss-Prot: P60174.3
Entrez Gene: 281543 Cow
Entrez Gene: 7167 Human
Entrez Gene: 21991 Mouse
Entrez Gene: 24849 Rat
Omim: 190450 Human
SwissProt: Q5E956 Cow
SwissProt: P54714 Dog
SwissProt: P60174 Human
磷酸丙糖异构酶（Triose-phosphate isomerase，通常简称为TPI或TIM）是一种能够催化丙糖磷酸异构体在二羟丙酮磷酸和D型甘油醛-3-磷酸之间转换。
磷酸丙糖异构酶在糖酵解中具有重要作用，对于有效的能量生成是必不可少的。磷酸丙糖异构酶被发现存在于几乎所有的生物体，包括哺乳动物、昆虫、真菌、植物和大多数细菌中。
如果人体缺乏磷酸丙糖异构酶，会导致严重的神经系统紊乱，该病症被称为磷酸丙糖异构酶缺乏症。
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Sample: Muscle (Mouse) Lysate at 40 ug
Primary: Anti-Triosephosphate isomerasee (SL4042R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 30 kD
Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti- Triosephosphate isomerase (SL4042R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-TPIS Polyclonal Antibody, Unconjugated(SL4042R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Positive control(blue): RSC96 cells
Isotype control(orange): Cell lines treated with rabbit IgG (SL0295P)instead of the primary antibody to confirm that primary antibody binding is specific. primary antibody(Green):Anti-Triosephosphate isomerase antibody(SL4042R),Concebtration: 5μg/10^6 cells
Secondary only control(light blue): Both cell lines treated with Goat Anti-rabbit IgG/FITC antibody (SL0295G-FITC) to confirm no background signal produced from secondary antibody alone.
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