The LASS (longevity-assurance homologue) family members, which are highly conserved among eukaryotes, function in ceramide synthesis. LASS6 (LAG1 homolog, ceramide synthase 6) may be involved in sphingolipid synthesis or its regulation.
Function:
May be involved in sphingolipid synthesis or its regulation
Subcellular Location:
Nucleus membrane; Multi-pass membrane protein (Potential). Endoplasmic reticulum membrane; Multi-pass membrane protein
Post-translational modifications:
Glycosylation on Asn-18 is not necessary for function
Similarity:
Contains 1 homeobox DNA-binding domain.
Contains 1 TLC (TRAM/LAG1/CLN8) domain.
SWISS:
Q6ZMG9
Gene ID:
253782
Database links:
Entrez Gene: 253782 Human
Entrez Gene: 241447 Mouse
Entrez Gene: 366065 Rat
SwissProt: Q6ZMG9 Human
SwissProt: Q8C172 Mouse
Unigene: 506829 Human
Unigene: 724377 Human
Unigene: 222222 Mouse
Unigene: 393211 Mouse
Unigene: 8190 Rat
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Paraformaldehyde-fixed, paraffin embedded (human pancreatic carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LASS6) Polyclonal Antibody, Unconjugated (SL5080R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LASS6) Polyclonal Antibody, Unconjugated (SL5080R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LASS6) Polyclonal Antibody, Unconjugated (SL5080R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-LASS6 Polyclonal Antibody, Unconjugated(SL5080R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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