Sample:
U937(Human) Lysate at 30 ug
Primary: Anti-phospho-CDKN1B (Thr157) (SL5228R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 25 kD
Sample:
Raji (Human) Lysate at 30 ug
Primary: Anti-phospho-CDKN1B (Thr157) (SL5228R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 25 kD
Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug
Lane 2: Thymus (Mouse) Lysate at 40 ug
Lane 3: Thymus (Rat) Lysate at 40 ug
Primary: Anti-phospho-CDKN1B (Thr157) (SL5228R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 25 kD
Sample:
MDA-MB-231(Human) Lysate at 30 ug
Primary: Anti-phospho-CDKN1B (Thr157) (SL5228R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 25 kD
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-CDKN1B (Thr157)) Polyclonal Antibody, Unconjugated (SL5228R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-CDKN1B (Thr157)) Polyclonal Antibody, Unconjugated (SL5228R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control (blue line): MCF7(fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice)
Primary Antibody (green line): Rabbit Anti-hospho-CDKN1B (Thr157) antibody (SL5228R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
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