Rabbit Anti-PIGR antibody | Sunlong Medical

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Hepatocellular carcinoma associated protein TB6; Phosphatidylinositol glycan, class R; PIgR; Poly Ig receptor; Polymeric immunoglobulin receptor; PIGR_HUMAN.

Cat:

SL6061R

Species Reactivity：

Human,(predicted: Mouse,Rat,Dog,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human PIGR:31-130/764<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: HepG2(Human) Cell Lysate at 30 ugPrimary: Anti-PIGR (SL6061R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 81 kDObserved band size: 131 kDSample: 293T(Human) Cell Lysate at 30 ugPrimary: Anti-PIGR (SL6061R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 81 kDObserved band size: 131 kDParaformaldehyde-fixed, paraffin embedded (Human hepatocellular carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PIGR) Polyclonal Antibody, Unconjugated (SL6061R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minuBlank control : 293T Cells (Blue line) Isotype control: SL0295P-AF647 (Orange line)Primary antibody: SL6061R-AF647 (Green line)COncentration: 2μg/10^6 cells,4℃ incubate 30min.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene is a member of the immunoglobulin superfamily. The encoded poly-Ig receptor binds polymeric immunoglobulin molecules at the basolateral surface of epithelial cells; the complex is then transported across the cell to be secreted at the apical surface. A significant association was found between immunoglobulin A nephropathy and several SNPs in this gene.[provided by RefSeq, Sep 2009]

Function:
This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Secretory component: Secreted.

Post-translational modifications:
N-glycosylation is not necessary for Ig binding.

Similarity:
Contains 5 Ig-like SLVtype (immunoglobulin-like)

SWISS:
P01833

Gene ID:
5284

Database links:

Entrez Gene: 5284 Human

Entrez Gene: 18703 Mouse

Omim: 173176 Human

SwissProt: P01833 Human

SwissProt: O70570 Mouse

Unigene: 497589 Human

Unigene: 276414 Mouse

多聚免疫球蛋白受体（pIgR）属Ⅰ型跨膜糖蛋白，可与多聚免疫球蛋白A和多聚免疫球蛋白M特异性结合，通过穿胞转运，将它们从上皮细胞基底侧膜转运到顶膜，并最终分泌到外分泌液中去. 在此过程中，多聚免疫球蛋白受体的细胞外段被水解，释放出与多聚免疫球蛋白A或多聚免疫球蛋白M相结合的细胞外段（又称为分泌成分）. 分泌成分是sIgA分子的重要组成部分，直接参与sIgA的粘膜防御功能，而且在被动粘膜免疫中也有重要作用. 多聚免疫球蛋白受体通过介导细胞内多聚免疫球蛋白的转运，可以在粘膜的腔面阻止病原体粘附，在上皮细胞内中和病毒，也可以将固有层内的抗原分泌出去. Picture

Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-PIGR (SL6061R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 81 kD
Observed band size: 131 kD

Paraformaldehyde-fixed, paraffin embedded (Human hepatocellular carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PIGR) Polyclonal Antibody, Unconjugated (SL6061R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minu

Blank control : 293T Cells (Blue line)
Isotype control: SL0295P-AF647 (Orange line)
Primary antibody: SL6061R-AF647 (Green line)
COncentration: 2μg/10^6 cells,4℃ incubate 30min.

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