Rabbit Anti-phospho-MCK10 (Tyr513) antibody

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MCK10 (phospho Y513); p-MCK10 (phospho Y513); CAK; CD167a; DDR1CD 167; CD167; Cell adhesion kinase; DDR 1; DDR; DDR1; Discoidin domain receptor; Discoidin receptor tyrosine kinase isoform a; EDDR 1; Epithelial discoidin domain receptor 1; Epithelial speci

Cat:

SL6269R

Species Reactivity：

Human,(predicted: Mouse,Rat,Pig,Cow,Horse,)

Immunogen：

KLH conjugated synthesised phosphopeptide derived from human MCK10 around the phosphorylation site of Tyr513:PA(p-Y)RL

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: HepG2(Human) Cell Lysate at 30 ugPrimary: Anti- phospho-MCK10 (Tyr513) (SL6269R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 99 kDObserved band size: 120 kDSample: K562(Human) Cell Lysate at 30 ugHela(Human) Cell Lysate at 30 ugPrimary: Anti- phospho-MCK10 (Tyr513) (SL6269R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 99 kDObserved band size: 120 kDSample: HepG2(Human) Cell Lysate at 30 ug Primary: Anti-phospho-MCK10 (SL6269R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 99 kD Observed band size: 120 kD Sample: K562(Human) Cell Lysate at 30 ugSiha(Human) Cell Lysate at 30 ugPrimary: Anti-phospho-MCK10 (SL6269R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 99 kDObserved band size: 120 kD

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI). In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain. This gene is located on chromosome 6p21.3 in proximity to several HLA class I genes. Alternative splicing of this gene results in multiple transcript variants.

Function:
Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.

Subunit:
Homodimer. Interacts (via PPxY motif) with WWC1 (via WW domains) in a collagen-regulated manner. Forms a tripartite complex with WWC1 and PRKCZ, but predominantly in the absence of collagen. Interacts (tyrosine phosphorylated) with SHC1. Interacts with SRC. Interacts with MYH9. Interacts with CDH1. Interacts with PTPN11. Interacts with NCK2.

Subcellular Location:
Isoform 1: Cell membrane; Single-pass type I membrane protein.
Isoform 2: Cell membrane; Single-pass type I membrane protein.
Isoform 3: Secreted (Potential).
Isoform 4: Cell membrane; Single-pass type I membrane protein.

Tissue Specificity:
Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.

Post-translational modifications:
Autophosphorylated in response to fibrillar collagen binding.

Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 1 F5/8 type C domain.
Contains 1 protein kinase domain.

SWISS:
Q08345

Gene ID:
780

Database links:

Entrez Gene: 780 Human

Entrez Gene: 12305 Mouse

Entrez Gene: 25678 Rat

Omim: 600408 Human

SwissProt: Q08345 Human

SwissProt: Q03146 Mouse

SwissProt: Q63474 Rat

Unigene: 631988 Human

Unigene: 5021 Mouse

Unigene: 7807 Rat

Picture

Sample:
K562(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-MCK10 (Tyr513) (SL6269R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 120 kD

Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-MCK10 (SL6269R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 120 kD

Sample:
K562(Human) Cell Lysate at 30 ug
Siha(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-MCK10 (SL6269R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 120 kD

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