Rabbit Anti-GPR55 antibody | Sunlong Medical

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GPCR GPR55; G protein coupled receptor 55; G protein-coupled receptor 55; G protein-coupled receptor 55; GPCR GPR55; GPR 55; GPR55; Probable G protein coupled receptor 55; GPR55_HUMAN.

Cat:

SL7686R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Dog,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human GPR55:141-240/319<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500Flow-Cyt=2ug/Test（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Human U87MG cell lysates Lane 2: Human U251 cell lysates Lane 3: Human U2os cell lysates Primary: Anti-GPR55 (SL7686R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 37 kDObserved band size: 37 kDSample: K562 Cell Lysate at 40 ug Primary: Anti- GPR55 (SL7686R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 35 kD Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR55) Polyclonal Antibody, Unconjugated (SL7686R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR55) Polyclonal Antibody, Unconjugated (SL7686R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control: RSC96 Cells(blue). Primary Antibody: Rabbit Anti-GPR55/AF488 Conjugated antibody (SL7686R-AF488), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR GPR55 is a member of this family (subfamily Orphan-A). It has been reported to be expressed in the brain, where mRNA transcripts were detected in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain. ESTs have been isolated from liver/spleen libraries.

Function:
May be involved in hyperalgesia associated with inflammatory and neuropathic pain. Receptor for L-alpha-lysophosphatidylinositol (LPI). LPI induces Ca(2+) release from intracellular stores via the heterotrimeric G protein GNA13 and RHOA. Putative cannabinoid receptor. May play a role in bone physiology by regulating osteoclast number and function.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed in the caudate nucleus and putamen, but not detected in the hippocampus, thalamus, pons cerebellum, frontal cortex of the brain or in the liver. Expressed in osteoclasts and osteoblasts.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
Q9Y2T6

Gene ID:
9290

Database links:

Entrez Gene: 9290 Human

Omim: 604107 Human

SwissProt: Q9Y2T6 Human

Unigene: 114545 Human

Picture

Sample: K562 Cell Lysate at 40 ug
Primary: Anti- GPR55 (SL7686R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD

Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR55) Polyclonal Antibody, Unconjugated (SL7686R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR55) Polyclonal Antibody, Unconjugated (SL7686R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control: RSC96 Cells(blue).
Primary Antibody: Rabbit Anti-GPR55/AF488 Conjugated antibody (SL7686R-AF488), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.

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