Rabbit Anti-BMP1 antibody | Sunlong Medical

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BMP 1; BMP-1; Bone morphogenetic protein 1; Mammalian tolloid protein; mTld; PCOLC; PCP; ProCollagen C endopeptidase; Procollagen C proteinase; TLD; BMP1_HUMAN.

Cat:

SL1281R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human BMP1:901-986/986

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Brain (Mouse) Lysate at 40 ugLung (Mouse) Lysate at 40 ugPrimary: Anti-BMP1 (SL1281R) at 1/300 dilutionSecondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilutionPredicted band size: 98 kDObserved band size: 98 kDSample: Liver (Mouse) Lysate at 40 ugPrimary: Anti-BMP1 (SL1281R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 98 kDObserved band size: 98 kDSample: Liver (Mouse) Lysate at 40 ugPrimary: Anti-BMP1 (SL1281R) at 1/300 dilutionSecondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilutionPredicted band size: 98 kDObserved band size: 98 kDTissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-BMP1 Polyclonal Antibody, Unconjugated(SL1281R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Bone morphogenetic protein 1 (BMP1) was first identified in osteogenic extracts of bone. It is an extracellular zinc endopeptidase, implicated in morphogenetic processes in a broad range of species. BMP1 is a member of the astacin family of metalloproteinases. The astacin family includes BMP1, astacin, meprin A and B, tolloid-like proteins, and choriolysin. BMP1 is involved in extracellular matrix (ECM) formation, suggesting that a functional link may exist between astacin metalloproteinases, growth factors, and cell differentiation and pattern formation during development. The name PCP reflects this enzyme’s involvement in the collagen deposition of growing bone. The enzymes known as the procollagen C and N proteinases (PCP and PNP) are involved in the processing of fibrillar procollagen precursors to mature collagens, which is an essential requirement for fibril formation. PCP cleaves the SLCterminus from procollagen, to allow the formation of mature, triplehelical collagen. The N-terminus is cleaved by the procollagen N-proteinase (PNP or ADAM-TS2). Defects in PNP have been linked to the skin disorder dermatosparaxis, and defects in BMP1 are thought to lead to aberrant collagen processing, and connective tissue disorders. Many forms of BMP1 have been reported, with varying truncation at the SLCterminus. The long form of BMP1 is most similar to the tolloid-like proteins, which have extra EGF-like and CUB domains.

Function:
Cleaves the SLCterminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD).

Tissue Specificity:
Ubiquitous.

Similarity:
Belongs to the peptidase M12A family. Contains 5 CUB domains. Contains 2 EGF-like domains.

SWISS:
P13497

Gene ID:
649

Database links:

Entrez Gene: 649 Human

Entrez Gene: 12153 Mouse

Entrez Gene: 83470 Rat

Omim: 112264 Human

SwissProt: P13497 Human

SwissProt: P19663 Mouse

Unigene: 1274 Human

Unigene: 27757 Mouse

Unigene: 9305 Rat

Picture

Sample: Liver (Mouse) Lysate at 40 ug
Primary: Anti-BMP1 (SL1281R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 98 kD

Sample: Liver (Mouse) Lysate at 40 ug
Primary: Anti-BMP1 (SL1281R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution
Predicted band size: 98 kD
Observed band size: 98 kD

Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-BMP1 Polyclonal Antibody, Unconjugated(SL1281R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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