Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
A431 (Human) Cell Lysate at 30 ug
Adrenal glands (Mouse) Lysate at 40 ug
Adrenal glands (Rat) Lysate at 40 ug
Uterus (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-c-Fos (Ser362) (SL12910R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62/46 kD
Observed band size: 46 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-c-Fos (Ser362) (SL12910R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-c-Fos (Ser362)) Polyclonal Antibody, Unconjugated (SL12910R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Generously provided by Markus Linder from Medical University Vienna as part of the Bioss Discovery Program. Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse osteosarcoma labeled with Anti-Phospho-c-Fos (Ser362) Polyclonal Antibody, Unconjugated (SL12910R) at 1:100 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-c-Fos (Ser362)) Polyclonal Antibody, Unconjugated (SL12910R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|