Rabbit Anti-phospho-FHOD1 (Thr1141) antibody

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FHOD1 (phospho T1141); p-FHOD1 (phospho T1141); FH1/FH2 domain containing protein; FH1/FH2 domain-containing protein 1; Fhod1; FHOD1_HUMAN; FHOS; FHOS1; Formin homolog overexpressed in spleen 1; Formin homology 2 domain containing 1; Formin homology 2 dom

Cat:

SL13159R

Species Reactivity：

Human,Rat,(predicted: Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthesised phosphopeptide derived from human FHOD1 around the phosphorylation site of Thr1141:RR(p-T)LK

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: 293T(Human) Cell Lysate at 30 ugPrimary: Anti- phospho-FHOD1 (Thr1141) (SL13159R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 126 kDObserved band size: 126 kDSample: K562(Human) Cell Lysate at 30 ugHela(Human) Cell Lysate at 30 ugPrimary: Anti- phospho-FHOD1 (Thr1141) (SL13159R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 126 kDObserved band size: 126 kDTissue/cell: Rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-phospho-FHOD1 Polyclonal Antibody, Unconjugated(SL13159R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-FHOD1 Polyclonal Antibody, Unconjugated(SL13159R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The limb deformity (ld) locus influences normal limb development and gives rise to alternative mRNAs that can translate into a family of protein products known as formins. Formins play a crucial role in cytoskeletal reorganization by influencing actin filament assembly. The temporal genetic hierarchy influencing normal limb development can deregulate and mediate mammalian developmental syndromes. FHOD1 induces the formation of and associates with bundled actin stress fibers in response to the activity of the Rho-ROCK cascade. It influences several cellular activities including cell migration, cytoskeletal arrangement, signal transduction and gene expression.

Function:
Required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation.

Subcellular Location:
Cytoplasm. Cytoplasm > cytoskeleton. Predominantly cytoplasmic.

Tissue Specificity:
Ubiquitous. Highly expressed in spleen.

Similarity:
Belongs to the formin homology family.
Contains 1 FH1 (formin homology 1) domain.
Contains 1 FH2 (formin homology 2) domain.
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.

SWISS:
Q9Y613

Gene ID:
29109

Database links:

Entrez Gene: 29109 Human

Omim: 606881 Human

SwissProt: Q9Y613 Human

Unigene: 95231 Human

Picture

Sample:
K562(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-FHOD1 (Thr1141) (SL13159R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 126 kD
Observed band size: 126 kD

Tissue/cell: Rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-phospho-FHOD1 Polyclonal Antibody, Unconjugated(SL13159R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-FHOD1 Polyclonal Antibody, Unconjugated(SL13159R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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