Rabbit Anti-TREM1 antibody | Sunlong Medical

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Triggering receptor expressed on myeloid cells 1; TREM 1; TREM-1; Triggering receptor expressed on monocytes 1; Triggering receptor TREM 1; Triggering receptor TREM1; Triggering-receptor TREM 1; Triggering-receptor TREM-1; Triggering-receptor TREM1; TREM1

Cat:

SL10306R

Species Reactivity：

Human,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from human TREM1:151-234/234<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample:hcclm3(Human) Cell Lysate at 30 ugK562(Human) Cell Lysate at 30 ugPrimary: Anti-TREM1 (SL10306R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 24 kDObserved band size: 21 kDParaformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TREM1) Polyclonal Antibody, Unconjugated (SL10306R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Primary Antibody:Rabbit anti-TREM1 antibody Supplier catalog number: SL10306R Supplier: Bioss Isotype Control Antibody: Rabbit IgG Secondary Antibody: F(ab’)2 fragment goat anti-rabbit IgG-PE Supplier: Jackson Lab. Positive control:U937(4% paraformaldehyde for 10 min at room temperature)Primary Antibody:Rabbit anti-TREM1 antibody Supplier catalog number: SL10306R Supplier: Bioss Isotype Control Antibody: Rabbit IgG Secondary Antibody: F(ab’)2 fragment goat anti-rabbit IgG-PE Supplier: Jackson Lab. Positive control:U937(4% paraformaldehyde for 10 min at room temperature)Primary Antibody:Rabbit anti-TREM1 antibodySupplier catalog number: SL10306RSupplier: BiossIsotype Control Antibody: Rabbit IgGSecondary Antibody: F(ab’)2 fragment goat anti-rabbit IgG-PESupplier: Jackson Lab. Positive control:U937(4% paraformaldehyde for 10 min at room temperature)

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011].

Function:
Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock.

Subunit:
Interacts with TYROBP/DAP12.

Subcellular Location:
Isoform 1: Cell membrane; Single-pass type I membrane protein (Potential). Isoform 2: Secreted (Potential).

Tissue Specificity:
Highly expressed in adult liver, lung and spleen than in corresponding fetal tissue. Also expressed in the lymph node, placenta, spinal cord and heart tissues. Expression is more elevated in peripheral blood leukocytes than in the bone marrow and in normal cells than malignant cells. Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Strongly expressed in acute inflammatory lesions caused by bacteria and fungi. Isoform 2 was detected in the lung, liver and mature monocytes.

Post-translational modifications:
Glycosylated.

Similarity:
Contains 1 Ig-like SLVtype (immunoglobulin-like) domain.

SWISS:
Q9NP99

Gene ID:
54210

Database links:

Entrez Gene: 54210 Human

Entrez Gene: 58217 Mouse

Omim: 605085 Human

SwissProt: Q9NP99 Human

SwissProt: Q9JKE2 Mouse

Unigene: 283022 Human

Unigene: 248352 Mouse

Picture

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TREM1) Polyclonal Antibody, Unconjugated (SL10306R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Primary Antibody:Rabbit anti-TREM1 antibody Supplier catalog number: SL10306R Supplier: Bioss Isotype Control Antibody: Rabbit IgG Secondary Antibody: F(ab’)2 fragment goat anti-rabbit IgG-PE Supplier: Jackson Lab. Positive control:U937(4% paraformaldehyde for 10 min at room temperature)

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