Rabbit Anti-HERC6 antibody | Sunlong Medical

Home > Product > Antibody > Rabbit Anti-HERC6 antibody

EC 6.3.2.; FLJ20637; HECT domain and RCC1 like domain containing protein 6; HECT domain and RCC1-like domain-containing protein 6; Hect domain and RLD 6; HERC 6; HERC6; HERC6_HUMAN; Potential ubiquitin ligase; Probable E3 ubiquitin protein ligase HERC6; P

Cat:

SL15463R

Species Reactivity：

Human,Mouse,Rat,(predicted: Pig,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human HERC6:751-850/1022

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Testis (Mouse) Lysate at 40 ugPrimary: Anti-HERC6 (SL15463R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 115 kDObserved band size: 115 kDSample: Embryo (Mouse) Lysate at 40 ugPrimary: Anti-HERC6 (SL15463R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 115 kDObserved band size: 115 kDSample:Brain (Mouse) Lysate at 40 ugPrimary: Anti-HERC6(SL15463R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 115kDObserved band size: 115kDSample: Lane 1: Mouse Placenta tissue lysates Lane 2: Mouse Testis tissue lysates Lane 3: Rat Placenta tissue lysates Lane 4: Rat Testis tissue lysatesLane 5: Human HL-60 cell lysates Lane 6: Human A431 cell lysates Lane 7: Human MOLT4 cell lysates Primary: Anti-HERC6 (SL15463R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 115 kDaObserved band size: 135 kDaParaformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HERC6) Polyclonal Antibody, Unconjugated (SL15463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

Picture

Sample:
Embryo (Mouse) Lysate at 40 ug
Primary: Anti-HERC6 (SL15463R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kD

Sample:Brain (Mouse) Lysate at 40 ug
Primary: Anti-HERC6(SL15463R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115kD
Observed band size: 115kD

Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Testis tissue lysates
Lane 3: Rat Placenta tissue lysates
Lane 4: Rat Testis tissue lysates
Lane 5: Human HL-60 cell lysates
Lane 6: Human A431 cell lysates
Lane 7: Human MOLT4 cell lysates
Primary: Anti-HERC6 (SL15463R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kDa
Observed band size: 135 kDa

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HERC6) Polyclonal Antibody, Unconjugated (SL15463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Feedback Wall