Rabbit Anti-GLUT2 antibody | Sunlong Medical

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liver; Glucose Transporter 2; Glucose Transporter GLUT2; Glucose transporter type 2; Glucose transporter type 2 liver; GLUT-2; GLUT2; GLUT 2; GTR2_HUMAN; SLC2A2; Solute carrier family 2 (facilitated glucose transporter) member 2; Solute carrier family 2 f

Cat:

SL10379R

Species Reactivity：

Human,Mouse,Rat,(predicted: Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human GLUT2:1-100/524<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestIF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Liver (Mouse) Lysate at 40 ugPrimary: Anti-GLUT2 (SL10379R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54 kDObserved band size: 54 kDSample: Kidney (Mouse) Lysate at 40 ugPrimary: Anti-GLUT2 (SL10379R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 54 kDObserved band size: 54 kDSample: Lane 1: Liver (Mouse) Lysate at 40 ugLane 2: Kidney (Mouse) Lysate at 40 ugLane 3: Pancreas (Mouse) Lysate at 40 ugPrimary: Anti-GLUT2 (SL10379R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 57/45 kDObserved band size: 47 kDTissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-GLUT2 Polyclonal Antibody, Unconjugated(SL10379R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingBlank control (blue line): Hep G2(fixed with 70% ethanol Overnight at 4℃). Primary Antibody (green line): Rabbit Anti-GLUT2 antibody (SL10379R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Glucose transporter 2 isoform is an integral plasma membrane glycoprotein of the liver, islet beta cells, intestine, and kidney epithelium. It mediates facilitated bidirectional glucose transport. Because of its low affinity for glucose, it has been suggested as a glucose sensor. [provided by RefSeq, Jul 2008].

Function:
Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Liver, insulin-producing beta cell, small intestine and kidney.

Post-translational modifications:
N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells.

DISEASE:
Defects in SLC2A2 are the cause of Fanconi-Bickel syndrome (FBS) [MIM:227810]. FBS is a rare, well-defined clinical entity, inherited in an autosomal recessive mode and characterized by hepatorenal glycogen accumulation, proximal renal tubular dysfunction, and impaired utilization of glucose and galactose.

Similarity:
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

SWISS:
P11168

Gene ID:
6514

Database links:

Entrez Gene: 6514 Human

Entrez Gene: 20526 Mouse

Entrez Gene: 25351 Rat

Omim: 138160 Human

SwissProt: P11168 Human

SwissProt: P14246 Mouse

SwissProt: P12336 Rat

Unigene: 167584 Human

Unigene: 18443 Mouse

Unigene: 89295 Rat

Picture

Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-GLUT2 (SL10379R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD

Sample:
Lane 1: Liver (Mouse) Lysate at 40 ug
Lane 2: Kidney (Mouse) Lysate at 40 ug
Lane 3: Pancreas (Mouse) Lysate at 40 ug
Primary: Anti-GLUT2 (SL10379R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57/45 kD
Observed band size: 47 kD

Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-GLUT2 Polyclonal Antibody, Unconjugated(SL10379R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Blank control (blue line): Hep G2(fixed with 70% ethanol Overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-GLUT2 antibody (SL10379R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

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