Rabbit Anti-CD361 antibody | Sunlong Medical

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EVI2B; CD361; D17S376; Ecotropic viral integration site 2B; Ecotropic viral integration site 2B protein homolog; EVDB; EVI 2B; EVI-2B; Evi2b; EVI2B protein; EVI2B_HUMAN; Protein EVI2B.

Cat:

SL14684R

Species Reactivity：

Human,Mouse,

Immunogen：

KLH conjugated synthetic peptide derived from human CD361:151-250/448<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti- CD361 (SL14684R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 46 kDObserved band size: 66 kDTissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-CD361 Polyclonal Antibody, Unconjugated(SL14684R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-CD361 Polyclonal Antibody, Unconjugated(SL14684R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: mosue embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-CD361 Polyclonal Antibody, Unconjugated(SL14684R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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EVI2B is a 448 amino acid protein which functions in the differentiation of melanocytes and keratinocytes. Lying within an intron of the Neurofibromin gene, the gene encoding EVI2B is transcribed from the telomere toward the centromere, which is opposite the transcription direction of the Neurofibromin gene. EVI2B is a single-pass transmembrane protein containing an extracellular domain with 4 glycosylation sites, a N-terminal signal peptide, a cytoplasmic hydrophilic domain and a hydrophobic transmembrane domain. Due to evidence suggesting that gene encoding the mouse homolog lies within a viral integration site that has been identitified in retrovirus-induced myeloid tumors, the gene encoding EVI2B may function as an oncogene in these tumor types. With expression in peripheral blood mononuclear cells, fibroblasts, bone marrow and EBSLVtransformed lymphoblastoid cell lines, EVI2B is implicated in leukemogenesis.

Subcellular Location:
Membrane.

Tissue Specificity:
Bone marrow, peripheral blood mononuclear cells, fibroblasts and Epstein-Barr virus-transformed lymphoblastoid cell lines.

SWISS:
P34910

Gene ID:
2124

Database links:

Entrez Gene: 2124 Human

Omim: 158381 Human

SwissProt: P34910 Human

Unigene: 5509 Human

Picture

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CD361 Polyclonal Antibody, Unconjugated(SL14684R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CD361 Polyclonal Antibody, Unconjugated(SL14684R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

Tissue/cell: mosue embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-CD361 Polyclonal Antibody, Unconjugated(SL14684R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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