Kv1.2 mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which K(+) ions may pass in accordance with their electrochemical gradient. Kv1.2 binds PDZ domains of DLG1, DLG2 and DLG4. The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
Function:
Mediates the voltage-dependent potassium ion
permeability of excitable membranes. Assuming opened or closed
conformations in response to the voltage difference across the
membrane, the protein forms a potassium-selective channel through
which potassium ions may pass in accordance with their
electrochemical gradient.
Subunit:
Heterotetramer of potassium channel proteins.
Subcellular Location:
Membrane; Multi-pass membrane protein
Similarity:
Belongs to the potassium channel family.
SWISS:
P16389
Gene ID:
3737
Database links:
Entrez Gene: 395117 Chicken
Entrez Gene: 3737 Human
Entrez Gene: 16490 Mouse
Entrez Gene: 25468 Rat
Omim: 176262 Human
SwissProt: P16389 Human
SwissProt: P63141 Mouse
SwissProt: P63142 Rat
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Sample:
Spinal cord (Mouse) Lysate at 40 ug
Primary: Anti-Kv1.2 (SL16865R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
Sample:
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-Kv1.2 (SL16865R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Kv1.2) Polyclonal Antibody, Unconjugated (SL16865R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Kv1.2) Polyclonal Antibody, Unconjugated (SL16865R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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