Rabbit Anti-TRIT1 antibody | Sunlong Medical

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2310075G14Rik; AI314189; AI314635; AV099619; FLJ20061; hGRO1; IPP transferase; IPPT; IPT; IPTase; Isopentenyl diphosphate:tRNA isopentenyltransferase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; MGC149242; MGC149243; mitochondrial;

Cat:

SL17134R

Species Reactivity：

Mouse,(predicted: Human,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human TRIT1:121-220/467

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Uterus(Mouse) Lysate at 40 ugPrimary: Anti-TRIT1(SL17134R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 48kDObserved band size: 53kDSample: Lung(Mouse)Lysate at 40 ugPrimary: Anti-TRIT1(SL17134R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 48kDObserved band size: 53kDSample: Kidney(Mouse)Lysate at 40 ugPrimary: Anti-TRIT1(SL17134R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilutionPredicted band size: 48kDObserved band size: 53kDParaformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRIT1) Polyclonal Antibody, Unconjugated (SL17134R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).

Subcellular Location:
Cytoplasm and Mitochondrion.

Similarity:
Belongs to the IPP transferase family.
Contains 1 matrin-type zinc finger.

SWISS:
Q9H3H1

Gene ID:
5962

Database links:

Entrez Gene: 5962 Human

Entrez Gene: 66966 Mouse

Entrez Gene: 362586 Rat

SwissProt: Q9H3H1 Human

SwissProt: Q80UN9 Mouse

Unigene: 356554 Human

Unigene: 235030 Mouse

Picture

Sample: Uterus(Mouse) Lysate at 40 ug
Primary: Anti-TRIT1(SL17134R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 48kD
Observed band size: 53kD

Sample: Lung(Mouse)Lysate at 40 ug
Primary: Anti-TRIT1(SL17134R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 48kD
Observed band size: 53kD

Sample: Kidney(Mouse)Lysate at 40 ug
Primary: Anti-TRIT1(SL17134R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 48kD
Observed band size: 53kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRIT1) Polyclonal Antibody, Unconjugated (SL17134R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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