The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]
Function:
Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9.
Subcellular Location:
Membrane.
Tissue Specificity:
T-helper type 1 lymphocyte (Th1)-specific.
Post-translational modifications:
O-glycosylated with core 1 or possibly core 8 glycans.
Similarity:
Belongs to the immunoglobulin superfamily. TIM family.
SWISS:
Q8VIM0
Gene ID:
171285
Database links:
Entrez Gene: 171285 Mouse
SwissProt: Q8VIM0 Mouse
Unigene: 72168 Mouse
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Sample:
Liver(Rat) Lysate at 40 ug
Primary: Anti-TIM 3 (SL8766R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 49 kD
Sample:
Thymus (Mouse) Lysate at 40 ug
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti-TIM 3 (SL8766R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 49 kD
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-TIM 3 (SL8766R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 49 kD
Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-TIM-3 Polyclonal Antibody, Unconjugated(SL8766R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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