Rabbit Anti-HTRA4 antibody | Sunlong Medical

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FLJ90724; High temperature requirement protean A4; High-temperature requirement factor A4; HTRA 4; HtrA serine peptidase 4; HTRA4; HTRA4_HUMAN; Probable serine protease HTRA4; Serine protease HTRA4.

Cat:

SL18101R

Species Reactivity：

Human,Mouse,Rat,(predicted: Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human HTRA4:251-350/476

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Protein: DU145(human) lyates at 30ug;Primary: Rabbit Anti-HTRA4(SL18101) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:49 kD Observed band size:50 kDSample: PSLC3 Cell (Human) Lysate at 30 ugPrimary: Anti- HTRA4 (SL18101R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 51 kDObserved band size: 51 kDProtein: muscle(mouse) lyates at 30ug;Primary: Rabbit Anti-HTRA4(SL18101) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:49 kD Observed band size:50 kDTissue/cell: Rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-HTRA4 Polyclonal Antibody, Unconjugated(SL18101R) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the HtrA family of proteases. The encoded protein contains a putative signal peptide, an insulin growth factor binding domain, a Kazal protease inhibitor domain, a conserved trypsin domain and a PDZ domain. Based on studies on other related family members, this enzyme may function as a secreted oligomeric chaperone protease to degrade misfolded secretory proteins. Other human HtrA proteins have been implicated in arthritis, tumor suppression, unfolded stress response, apoptosis, and aging. [provided by RefSeq, Oct 2008]

Function:
Serine protease.

Subcellular Location:
Secreted.

Similarity:
Belongs to the peptidase S1B family.
Contains 1 IGFBP N-terminal domain.
Contains 1 Kazal-like domain.
Contains 1 PDZ (DHR) domain.

SWISS:
P83105

Gene ID:
20620

Database links:

Entrez Gene: 20620 Human

Entrez Gene: 330723 Mouse

Omim: 610700 Human

SwissProt: P83105 Human

SwissProt: A2RT60 Mouse

Unigene: 661014 Human

Unigene: 334452 Mouse

Picture

Sample:
PSLC3 Cell (Human) Lysate at 30 ug
Primary: Anti- HTRA4 (SL18101R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD

Protein: muscle(mouse) lyates at 30ug;
Primary: Rabbit Anti-HTRA4(SL18101) at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000;
Predicted band size:49 kD
Observed band size:50 kD

Tissue/cell: Rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-HTRA4 Polyclonal Antibody, Unconjugated(SL18101R) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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