Mex3d is a ubiquitously expressed protein. Due to alternative splicing events truncating the N-terminus, a variant form of Mex3d exists and is known as TINO.
Function:
RNA binding protein, may be involved in post-transcriptional regulatory mechanisms.
Subcellular Location:
Cytoplasm Nucleus
Tissue Specificity:
Ubiquitously expressed in all the cell lines and tissues tested.
Post-translational modifications:
Phosphorylated
Similarity:
Contains 2 KH domains.
Contains 1 RING-type zinc finger.
SWISS:
Q86XN8
Gene ID:
399664
Database links:
Entrez Gene: 399664 Human
Omim: 611009 Human
SwissProt: Q86XN8 Human
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Sample: U251 Cell (Human) Lysate at 40 ug
Primary: Anti-MEX3D/RNF193 (SL18822R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 62 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF193) Polyclonal Antibody, Unconjugated (SL18822R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF193) Polyclonal Antibody, Unconjugated (SL18822R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF193) Polyclonal Antibody, Unconjugated (SL18822R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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