Sample:
Lane 1: Lymph node (Mouse) Lysate at 40 ug
Lane 2: Esophagus (Mouse) Lysate at 40 ug
Lane 3: K562 (Human) Cell Lysate at 30 ug
Lane 4: Molt-4 (Human) Cell Lysate at 30 ug
Lane 5: Siha (Human) Cell Lysate at 30 ug
Primary:
Anti-CDK2 (SL10726R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-CDK2 (SL10726R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Sample:
MCF-7 Cell (Human) Lysate at 30 ug
Primary: Anti-CDK2 (SL10726R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 30 kD
Sample: testis (Mouse) Lysate at 40 ug
Primary: Anti-CDk2(SL10726R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Blank control (Black line): U87MG(Black).
Primary Antibody (green line): Rabbit Anti-CDK2 antibody (SL10726R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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