Rabbit Anti-NHP2L2 antibody

Home > Product > Antibody > Rabbit Anti-NHP2L2 antibody

BPX; Brain specific gene BPX; Brain specific protein, X linked; Brain-specific protein; MGC26243; Nap1l2; NP1L2_HUMAN; Nucleosome assembly protein 1 like 2; Nucleosome assembly protein 1-like 2; X-linked.

Cat:

SL19237R

Species Reactivity：

Mouse,Rat,(predicted: Human,Dog,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human NHP2L2:141-340/460

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:1000-3000ELISA=1:2000-5000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Brain (Mouse) Lysate at 40 ugPrimary: Anti-NHP2L2n (SL19237R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 53 kDObserved band size: 53 kDSample: U251(Human) Cell Lysate at 30 ugPrimary: Anti-NHP2L2n (SL19237R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 53 kDObserved band size: 53 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NHP2L2) Polyclonal Antibody, Unconjugated (SL19237R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NHP2L2) Polyclonal Antibody, Unconjugated (SL19237R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

prev next

Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
Feedback Wall

The protein encoded by this intronless gene is a member of the nucleosome assembly protein (NAP) family. The encoded protein represents a class of tissue-specific factors that interact with chromatin to regulate neuronal cell proliferation. [provided by RefSeq, Jan 2011]

Function:
Acidic protein which may be involved in interactions with other proteins or DNA.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the nucleosome assembly protein (NAP) family.

SWISS:
Q9ULW6

Gene ID:
4674

Database links:

Entrez Gene: 4674 Human

Entrez Gene: 17954 Mouse

Entrez Gene: 317247 Rat

Omim: 300026 Human

SwissProt: Q9ULW6 Human

SwissProt: P51860 Mouse

Unigene: 6636 Human

Unigene: 740565 Human

Unigene: 388694 Mouse

Unigene: 43670 Rat

Picture

Sample:
U251(Human) Cell Lysate at 30 ug
Primary: Anti-NHP2L2n (SL19237R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NHP2L2) Polyclonal Antibody, Unconjugated (SL19237R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NHP2L2) Polyclonal Antibody, Unconjugated (SL19237R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Feedback Wall