Home > Product > Antibody > Rabbit Anti-NRBP2 antibody
BC011468; DKFZp434P086; MGC12117; MGC138699; MGC18994; NRBP2; NRBP2_HUMAN; Nuclear receptor binding protein 2; Nuclear receptor-binding protein 2; PP9320; Transformation related gene 16 protein; Transformation-related gene 16 protein; transfor
Cat:
SL19344R
Species Reactivity:
Human,Mouse,Rat,(predicted: Cat,)
Immunogen:
KLH conjugated synthetic peptide derived from human NRBP2:401-501/501
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Sample: Cerebellum (mouse) Lysate at 40 ugPrimary: Anti-NRBP2(SL19344R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 58 kDObserved band size: 58 kDSample: brain (mouse) Lysate at 40 ugPrimary: Anti-NRBP2(SL19344R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 58 kDObserved band size: 58 kDTissue/cell: Human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min; Incubation: Anti-NRBP2 Polyclonal Antibody, Unconjugated(SL19344R) 1:400, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) stainingTissue/cell: Rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min; Incubation: Anti-NRBP2 Polyclonal Antibody, Unconjugated(SL19344R) 1:400, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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Sample: Cerebellum (mouse) Lysate at 40 ug
Primary: Anti-NRBP2(SL19344R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Sample: brain (mouse) Lysate at 40 ug
Primary: Anti-NRBP2(SL19344R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Tissue/cell: Human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min;
Incubation: Anti-NRBP2 Polyclonal Antibody, Unconjugated(SL19344R) 1:400, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
Tissue/cell: Rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37∩ for 20 min;
Incubation: Anti-NRBP2 Polyclonal Antibody, Unconjugated(SL19344R) 1:400, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
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