Rabbit Anti-PPM1E antibody | Sunlong Medical

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Ca(2+)/calmodulin-dependent protein kinase phosphatase N; CaMKN; CaMKP-N; CaMKP-nucleus; KIAA1072; Nuclear calmodulin dependent protein kinase phosphatase; Partner of PIX 1; Partner of PIX-alpha; Partner of PIXA; POPX1; PP2CH; Ppm1e; PPM1E_HUM

Cat:

SL21063R

Species Reactivity：

Mouse,(predicted: Human,Rat,Chicken,Pig,Cow,Horse,Zebrafish,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human PPM1E:321-420/764

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-PPM1E (SL21063R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 85 kDObserved band size: 85 kDSample: Olfactory buib(Mouse) Lysate at 40 ugHippocampus (Mouse) Lysate at 40 ugPrimary: Anti- PPM1E (SL21063R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 85 kDObserved band size: 85 kDSample: Hippocampus (Mouse) Lysate at 40 ugPrimary: Anti-PPM1E (SL21063R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 85 kDObserved band size: 85 kDParaformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPM1E) Polyclonal Antibody, Unconjugated (SL21063R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the PPM family of serine/threonine-protein phosphatases. The encoded protein is localized to the nucleus and dephosphorylates and inactivates multiple substrates including serine/threonine-protein kinase PAK 1, 5'-AMP-activated protein kinase (AMPK) and the multifunctional calcium/calmodulin-dependent protein kinases. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]

Function:
Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2 (By similarity). Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42.

Subcellular Location:
Nucleus. Cytoplasm. A truncated form, major form, with the SLCterminal part missing, is mostly found in the cytoplasm and a little in the nucleus. The full length, minor form, is found in the nucleus.

Similarity:
Belongs to the PP2C family.
Contains 1 PP2SLClike domain.

SWISS:
Q8WY54

Gene ID:
22843

Database links:

Entrez Gene: 22843 Human

SwissProt: Q8WY54 Human

Unigene: 245044 Human

Picture

Sample:
Olfactory buib(Mouse) Lysate at 40 ug
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti- PPM1E (SL21063R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 85 kD
Observed band size: 85 kD

Sample:
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti-PPM1E (SL21063R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 85 kD
Observed band size: 85 kD

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPM1E) Polyclonal Antibody, Unconjugated (SL21063R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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