Rabbit Anti-TRIM23 antibody

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ADP ribosylation factor domain protein 1; ARF domain protein 1; ARFD1; GTP binding protein ARD 1; TRI23_HUMAN; N-acetyltransferase ARD1 human homolog of; N-acetyltransferase homolog of S. cerevisiae ARD1; TRIM23; Tripartite motif protein TRIM23.

Cat:

SL20285R

Species Reactivity：

Human,Rat,(predicted: Mouse,Dog,Pig,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human TRIM23:201-300/574

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Hela (Human) Cell Lysate at 40 ugPrimary: Anti-TRIM23(SL20285R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 64 kDObserved band size: 64 kDSample: 293T (Human)Cell Lysate at 40 ugPrimary: Anti-TRIM23(SL20285R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 64 kDObserved band size: 64 kDSample: A549 Cell (Human) Lysate at 40 ugPrimary: Anti-TRIM23 (SL20285R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 64 kDObserved band size: 64 kDParaformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRIM23) Polyclonal Antibody, Unconjugated (SL20285R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein is also a member of the ADP ribosylation factor family of guanine nucleotide-binding family of proteins. Its carboxy terminus contains an ADP-ribosylation factor domain and a guanine nucleotide binding site, while the amino terminus contains a GTPase activating protein domain which acts on the guanine nucleotide binding site. The protein localizes to lysosomes and the Golgi apparatus. It plays a role in the formation of intracellular transport vesicles, their movement from one compartment to another, and phopholipase D activation. Three alternatively spliced transcript variants for this gene have been described. [provided by RefSeq, Jul 2008]

Function:
Acts as an E3 ubiquitin-protein ligase. In the presence of the human cytomegalovirus (HCMV) protein UL144, participates in 'Lys-63'-linked auto-ubiquitination of TRAF6 resulting in the virally controlled activation of NF-kappa-B at early time of infection. The SLCterminus can act as an allosteric activator of the cholera toxin catalytic subunit.

Subunit:
Interacts with PSCD1. Interacts with human cytomegalovirus protein UL144; this interaction might causes auto-ubiquitination of TRAF6, leading to NF-kappaB activation.

Subcellular Location:
Endomembrane system. Golgi apparatus membrane. Lysosome membrane. Note: Membrane associated with the Golgi complex and lysosomal structures.

Similarity:
In the SLCterminal section; belongs to the small GTPase superfamily. Arf family.
Contains 1 B box-type zinc finger.

SWISS:
P31286

Gene ID:
373

Database links:

Entrez Gene: 373 Human

Entrez Gene: 81003 Mouse

Entrez Gene: 81002 Rat

SwissProt: P31286 Human

SwissProt: Q8BGX0 Mouse

SwissProt: P31287 Rat

Picture

Sample: 293T (Human)Cell Lysate at 40 ug
Primary: Anti-TRIM23(SL20285R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 64 kD

Sample: A549 Cell (Human) Lysate at 40 ug
Primary: Anti-TRIM23 (SL20285R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 64 kD

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRIM23) Polyclonal Antibody, Unconjugated (SL20285R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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