Rabbit Anti-iNos/Nos-2 antibody

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i NOS; Nitric Oxide Synthase, Inducible; HEP NOS; Hepatocyte NOS; HEPNOS; Inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; INOS; Inosl; MAC NOS; Macrophage NOS; Nitric oxide synthase 2 inducible macrophage; Nitric oxide synthase 2A (

Cat:

SL20601R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Pig,Horse,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human iNos/Nos-2:1051-1153/1153

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: MCF-7 Cell (Human) Lysate at 40 ugPrimary: Anti-iNos/Nos-2(SL20601R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 130kDObserved band size: 125kDSample: DU145 Cell (Human) Lysate at 40 ugPrimary: Anti-iNos/Nos-2(SL20601R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 130kDObserved band size: 125kD Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (iNos) Polyclonal Antibody, Unconjugated (SL20601R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (iNos) Polyclonal Antibody, Unconjugated (SL20601R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control (blue line): Hep G2(fixed with 70% ethanol Overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature). Primary Antibody (green line): Rabbit Anti-iNOS antibody (SL20601R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase SLCstimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).

Function:
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

Subunit:
Homodimer. Binds SLC9A3R1.

Tissue Specificity:
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.

Similarity:
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.

SWISS:
P35228

Gene ID:
4843

Database links:

Entrez Gene: 4843 Human

Entrez Gene: 18126 Mouse

Entrez Gene: 24599 Rat

Omim: 163730 Human

SwissProt: P35228 Human

SwissProt: P29477 Mouse

SwissProt: Q06518 Rat

Unigene: 709191 Human

Unigene: 2893 Mouse

Unigene: 10400 Rat

合成与降解（Synthesis and Degradation）
催化生物体内一氧化氮（NO）生成的酶。分神经型一氧化氮合成的酶（nNOS or NOS-1）、诱导型一氧化氮合成的酶（iNOS or NOS-2）、内皮型一氧化氮合成的酶（eNOS or NOS-3）。 Picture

Sample: DU145 Cell (Human) Lysate at 40 ug
Primary: Anti-iNos/Nos-2(SL20601R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130kD
Observed band size: 125kD

Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (iNos) Polyclonal Antibody, Unconjugated (SL20601R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (iNos) Polyclonal Antibody, Unconjugated (SL20601R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Blank control (blue line): Hep G2(fixed with 70% ethanol Overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature).
Primary Antibody (green line): Rabbit Anti-iNOS antibody (SL20601R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

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