Sample:
Lung Cell (Mouse) Lysate at 40 ug
Primary: Anti-NKG2D (SL20661R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD
Sample:
Stomach (Mouse) Lysate at 40 ug
Primary: Anti- NKG2D (SL20661R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti- NKG2D (SL20661R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD
Blank control (blue line): Mouse thymus cells (fixed with 70% methanol (Overnight at 4℃)).
Primary Antibody (green line): Rabbit Anti-NKG2D antibody (SL20661R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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