Rabbit Anti-Semaphorin 3F antibody

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Semaphorin-3F; Semaphorin3F; Sema III/F; Sema IV; sema3F; SemaIII/F; SemaIV; Semaphorin IV; SemaphorinIV; SEM3F_HUMAN.

Cat:

SL23306R

Species Reactivity：

Mouse,Rat,(predicted: Human,Chicken,Dog,Pig,Cow,Rabbit,Sheep,)

Immunogen：

KLH conjugated synthetic peptide derived from human Sema3F:201-300/785

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lung (Mouse) Lysate at 40 ugCerebrum (Mouse) Lysate at 40 ugPrimary: Anti-Semaphorin 3F (SL23306R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 88 kDObserved band size: 88 kDSample: Cerebrum(Mouse) Cell Lysate at 40 ugPrimary: Anti-Semaphorin 3F (SL23306R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 88 kDObserved band size: 88 kDSample: Cerebrum (Mouse) Lysate at 40 ugPrimary: Anti-Semaphorin 3F (SL23306R) at 1/500 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 88 kDObserved band size: 88 kDSample: Lane 1: Mouse Brain Lysates Lane 2: Rat Brain Lysates Primary: Anti-Semaphorin 3F (SL23306R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 88kDaObserved band size: 88 kDaSample: Hypophysis (Mouse) Lysate at 40 ugPrimary: Anti- Semaphorin 3F (SL23306R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 88 kDObserved band size: 88 kD

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues(Kolodkin et al., 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al., 1993 [PubMed 1682908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al., 1995 [PubMed7637782]), a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.

Function:
May play a role in cell motility and cell adhesion.

Subunit:
Belongs to the semaphorin family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Sema domain.

Subcellular Location:
Secreted.

Tissue Specificity:
Expressed abundantly but differentially in a variety of neural and nonneural tissues. There is high expression in mammary gland, kidney, fetal brain, and lung and lower expression in heart and liver.

SWISS:
Q13275

Gene ID:
1285

Database links:

Entrez Gene: 1285 Human

Entrez Gene: 20350 Mouse

Entrez Gene: 315996 Rat

Omim: 601124 Human

SwissProt: Q13275 Human

SwissProt: O88632 Mouse

Unigene: 32981 Human

Unigene: 12903 Mouse

Unigene: 205616 Rat

Picture

Sample:
Cerebrum(Mouse) Cell Lysate at 40 ug
Primary: Anti-Semaphorin 3F (SL23306R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kD
Observed band size: 88 kD

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Semaphorin 3F (SL23306R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kD
Observed band size: 88 kD

Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Rat Brain Lysates
Primary: Anti-Semaphorin 3F (SL23306R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88kDa
Observed band size: 88 kDa

Sample:
Hypophysis (Mouse) Lysate at 40 ug
Primary: Anti- Semaphorin 3F (SL23306R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kD
Observed band size: 88 kD

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