Rabbit Anti-NR2F1/NR2F2 antibody

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Apolipoprotein AI regulatory protein 1; ARP1; COUP TF1; COUP TF2; COUP transcription factor 2; Chicken ovalbumin upstream promoter 1; COT1_HUMAN; COUP transcription factor 1; COUP transcription factor I; COUP-TF I; COUP-TF1; EAR-3; EAR3; ERBAL3; NR2F1; NR

Cat:

SL22140R

Species Reactivity：

Human,Mouse,Rat,(predicted: Chicken,Dog,Pig,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human NR2F1/NR2F2:111-210/414

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

IHC-P=1:100-500IHC-F=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NR2F1) Polyclonal Antibody, Unconjugated (SL22140R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NR2F1) Polyclonal Antibody, Unconjugated (SL22140R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NR2F1) Polyclonal Antibody, Unconjugated (SL22140R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat embryonic kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NR2F1) Polyclonal Antibody, Unconjugated (SL22140R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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NR2F2 is involved in the regulation of the apolipoprotein A-I gene transcription. It binds to DNA site A as a homodimer. It belongs to the nuclear hormone receptor family, NR2 subfamily. It has roles in angiogenesis, vascular remodeling and heart development.

Function:
Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.

Subunit:
Binds DNA as dimer; homodimer and probable heterodimer with NR2F6. Interacts with COPS2.

Subcellular Location:
Nucleus (Probable).

Similarity:
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain.

SWISS:
P24468

Gene ID:
7026

Database links:

Entrez Gene: 281945 Cow

Entrez Gene: 7026 Human

Entrez Gene: 11819 Mouse

Entrez Gene: 113984 Rat

Omim: 107773 Human

SwissProt: Q9TTR7 Cow

SwissProt: P24468 Human

SwissProt: P43135 Mouse

SwissProt: O09018 Rat

Unigene: 347991 Human

Unigene: 17815 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NR2F1) Polyclonal Antibody, Unconjugated (SL22140R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NR2F1) Polyclonal Antibody, Unconjugated (SL22140R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat embryonic kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NR2F1) Polyclonal Antibody, Unconjugated (SL22140R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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