Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha 2A, alpha 2B, and alpha 2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both the alpha 2A and alpha 2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the alpha 2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha 2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes alpha 2A subtype and it contains no introns in either its coding or untranslated sequences. [provided by RefSeq].
Function:
Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol.
Subcellular Location:
Cell membrane.
Similarity:
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily.
ADRA2A sub-subfamily.
SWISS:
P08913
Gene ID:
150
Database links:
Entrez Gene: 150 Human
Entrez Gene: 11551 Mouse
Entrez Gene: 25083 Rat
Entrez Gene: 266750 Zebrafish
Omim: 104210 Human
SwissProt: P08913 Human
SwissProt: Q01338 Mouse
SwissProt: P22909 Rat
SwissProt: Q1L8Y8 Zebrafish
SwissProt: Q90WY4 Zebrafish
Unigene: 249159 Human
Unigene: 235195 Mouse
Unigene: 170171 Rat
Unigene: 30878 Zebrafish
| Picture |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADRA2) Polyclonal Antibody, Unconjugated (SL1062R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-ADRA2 Polyclonal Antibody, Unconjugated(SL1062R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining
|
|
|
