Rabbit Anti-AQP2 antibody | Sunlong Medical

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ADH water channel; AQP 2; AQP-2; AQP CD; AQPCD; Aquaporin 2 collecting duct; Aquaporin CD; Aquaporin2; Aquaporine 2; Collecting duct water channel protein; MGC34501; Water channel protein for renal collecting duct; WCH CD; WCHCD.

Cat:

SL0261R

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from human AQP2:101-200/271<Extracellular>

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Heart (Mouse) Lysate at 40 ugPrimary: Anti-Aquaporin 2 (SL0261R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 30 kDObserved band size: 35 kDSample: Kidney (Mouse) Lysate at 40 ug293T(Human) Cell Lysate at 30 ugPrimary: Anti- Aquaporin 2 (SL0261R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 30 kDObserved band size: 32 kDTissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min; Incubation: Anti-AQP-2 Polyclonal Antibody, Unconjugated(SL0261R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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Unit:

Price: $220.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant, and recessive forms of nephrogenic diabetes insipidus.

Function:
Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

Subcellular Location:
Apical cell membrane. Cytoplasmic vesicle membrane. Shuttles from vesicles to the apical membrane.

Tissue Specificity:
Expressed in renal collecting tubules.

Post-translational modifications:
Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.

DISEASE:
Defects in AQP2 are the cause of diabetes insipidus nephrogenic autosomal (ANDI) [MIM:125800]; also known as diabetes insipidus nephrogenic type 2. ANDI is caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. It is characterized by excessive water drinking (polydypsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. Inheritance can be autosomal dominant or recessive.

Similarity:
Belongs to the MIP/aquaporin (TC 1.A.8) family.

SWISS:
P41181

Gene ID:
359

Database links:

Entrez Gene: 359 Human

Entrez Gene: 11827 Mouse

Entrez Gene: 25386 Rat

Omim: 107777 Human

SwissProt: P41181 Human

SwissProt: P51282 Mouse

SwissProt: P34080 Rat

Unigene: 130730 Human

Unigene: 20206 Mouse

Unigene: 90076 Rat

通道蛋白（Channel Protein）
水通道蛋白-2特异地存在于肾脏集合管，受血管加压素调节。有研究证明，AQP2(水通道蛋白-2)在糖尿病肾脏集合管表达增强.
在1型糖尿病动物模型的水转运中尤其有意义。研究认为：AQP-2 奠定了糖尿病大鼠肾脏对水重吸收的基础，而且发现1型糖尿病病人的 AQP-2随血糖控制而减少。 Picture

Sample:
Kidney (Mouse) Lysate at 40 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti- Aquaporin 2 (SL0261R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 32 kD

Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,SLC0005) at 37℃ for 20 min;
Incubation: Anti-AQP-2 Polyclonal Antibody, Unconjugated(SL0261R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(SLC0010) staining

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